[Aguascalientes] Neurohormone Gonadotropin-Releasing Hormone agonist and neuroregeneration in spinal cord injury model in animals
Universidad Autonoma de Aguascalientes
Department of Physiology and Pharmacology. Department of Basic Science (lab 3, building 202). Universidad Autonoma de Aguascalientes. Av. Universidad # 940. Col. Ciudad Universitaria. CP 20131. Aguascalientes. Ags, Mexico
Dr. Marin Gerardo Rodriguez
Dr. José Luis Quintanar Stephano, PhD
English and spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
Gonadotropin-Releasing Hormone (GnRH) has neuromodulatory roles in central and peripheral nervous systems. It has excitatory effects on rat cerebral cortical neurons and up-regulates the Ca 2+ channel density of sympathetic neurons. It has been reported that GnRH has neurotrophic effects on neurite outgrowth and neurofilament protein expression in cultured cerebral cortical neurons of rat embryos. Also, it has been described that the GnRH receptor and its mRNA exist in spinal cord neurons of embryos and adult rats. In cultured cerebral cortical neurons of rat embryos, GnRH has increased the number and length of neuritis and the cytoskeletal neurofilament expression. Studies suggest that GnRH could operate as a neurotrophic factor that acts in the hippocampus where GnRH treatments induce changes in the spine’s synapse density.
What is the aim of the project?
- Determine the changes in the diameters (micreometer [µm]) of the regenerated myelinated fibers and the nerve conduction velocity of the GnRH –treated rats - Compare the results of the GnRH–treated rats with the normal saline– treated ones.
What techniques and methods are used?
- Techniques of neurovascular microsurgery with Wistar rats such as hypophysectomy, a carotid occlusion. - Special immunohistochemically stain like enzyme- linked immunosorbent assay (ELISA) with different tissues. - Molecular biology techniques suchs as Polymerase Chain Reaction (PCR) and Western Blot.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will first read about the role of the GnRH on maintaining the homeostasis. Then to observe how to carry out Protein Chain Reaction (PCR) and Western Blot techniques and he/she will acquire basic research laboratory skills such as care and management of laboratory animals and basic laboratory equipment. When the student is ready, he/she will join to one of the projects in the lab by making special immunohistochemically stains with different tissues in the context of neural regeneration.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, there will be readings and seminars on spinal cord injury and degrees of spinal cord injury, mechanisms of action of GnRH in the body and how this can help the recovery of the patient with spinal cord injury and what alterative exist aqueous for the patient with spinal cord injury.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It is required for the student to have manual dexterity, practical work skills, and knowledge of physiology of the nervous system.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
- Functional and structural recovery of the injured spinal cord in rats treated with Gonadotropin- releasing hormone. (2015) Calderón-Vallejo D.; Quintanar-Stephano A; Hernández-Jasso I.; Jiménez- Hernández V; Ruiz-Ornelas J; Jiménez I; Quintanar J.L. Neurochemical Research. 40 (3): 455-462. DOI: 10.1007/s11064-014-1486-9.
- Leuprolide acetate; a GnRH agonist; induces structural and functional recovery of injured spinal cord in rat. (2015). Carmen Díaz-Galindo; Beatriz Gómez-González; Eva Salinas; Denisse Calderón- Vallejo; Irma Hernández-Jasso; Eduardo Bautista and J. Luis Quintanar. Neural Regeneration Research. 10(11):1819-1824.
- Leuprolide acetate inhibits spinal cord inflammatory response in experimental autoimmune encephalomyelitis by suppressing NF-ƒÈB activation. (2015). Guzman-Soto I; Salinas E and Quintanar J. Luis. Neuroimmunomodulation. DOI: 10.1159/000438927