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Principles and approaches of laboratory diagnostics in medical microbiology
Bosnia and Herzegovina (BoHeMSA) - University of Mostar, Mostar
Institute for microbiology and molecular diagnostics, University Clinical Hospital Mostar
Maja Ostojic MD, PhD
Tutor(s): Tanja Petrovic MD; Sanja Jakovac MD
English, Croatian, Bosnian, Serbian
Type of Research Project
- Basic science
What is the background of the project?
Medical Microbiology is one the elementary subjects of medical education. Students all around the globe attend lectures and learn importance of microorganisms in pathological and physiological processes of organism. However, we believe that no theoretical background is useful without practical knowledge. All tests mastered during this exchange are fundamental for diagnosis in clinical practice. Ever since the discovery of bacteria and virus it has been a routine to identify microorganism as a source of infection. Today we count numerous different diseases classified only due to a variety of test that indicate the pathogen itself.
What is the aim of the project?
Main aim of the project is to develop awareness and importance of microbiome, to understand bacterial resistance to certain antimicrobial drugs and to understand importance of vaccines.
What techniques and methods are used?
Some of the techniques and methods that are going to be used during the exchange are: • Bacterial staining • Microscopy • Cultivation and biochemical identification • Serological tests • Antimicrobial susceptibility testing • Rapid diagnostics tests • Viral cultivation in embryonated chicken eggs • Mycology identification. The project would be implemented at the Department of Medical Microbiology for a period of 4 weeks, 6 hours per day. The project would consist short lectures, literary research and practical work. It would be also possible to visit some associations and get acquainted with their work. Lectures and practical work will be aligned, so it is desirable to have continuous attendance and student activity. First week student will go through short introduction to basics of microbiology. After introduction research starts with basics of bacteriology and highly specific tests of their identification (exp. student will grow bacetrial colonies on platelets etc.).Once mastering bacteriology student will start the virology tests with emphasis on embryonated chicken egg cultivation. Final step will include microscoping fungi and basics of mycology. At the end of the project, students are required to make a short essay on everything they have learned in the past 4 weeks.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Student should master these during exchange: • to sterilize microbial dishes (loop, tube etc.) using specific devices (such as autoclave) • to stain bacteria by Gram with assistance of supervisor • to inoculate material in to culture media with assistance of supervisor • to examine bacterial colonies (recognize typical bacterial behaviour) • to make disk-diffusion method (specific antibiograms) • to do agglutination test • to cultivate viruses into embryonated chicken eggs • to do biochemical analysis of bacteria • microscopy bacteria and parasites (to identify species by their morphological characteristics) • to understand the role of microbiome
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be theoretical teaching through short lectures, seminars, workshops and preliminary readings of recent literature.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Imunology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Kathleen Park Talaro; Barry Chess; Foundations in microbiology; 2018
- A clinician's guide to microbiome analysis. Claesson MJ; Clooney AG; O'Toole PW 2017 Oct;14(10):585-595. doi: 10.1038/nrgastro.2017.97.
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