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Evaluation of the metabolite effects of purine nucleotides on the activity of antioxidant system of enzymes with sympathetic hyperactivation.
Kazakhstan (KazMSA) - Semey State Medical University, SSMU, Semey, Kazakhstan
Department of Biochemistry and Chemical Disciplines of the Semey Medical University, SMU, Semey, Kazakhstan, str. Abay 103., 071400
c.m.s., Smailova Zhanargul Kayirgaliyevna.
c.b.s., Associate Professor, Olzhaeva Rauza Romanovna. Teacher of the Department of Biochemistry and Chemical Disciplines of the State Medical University of Semey, Sovetov Bakhytbek Sovetovich
Required: English Accepted: English, Kazakh, Russian.
Type of Research Project
- Basic science
What is the background of the project?
We are interested to determine activation of the sympathoadrenal system, which aggravates the course of coronary heart disease. A high level of catecholamines is a risk factor for the development of recurrent myocardial infarction and sudden death. Sympathetic hyperactivation is accompanied by enhanced formation of products of non-enzymatic oxidation of adrenaline. Simultaneously, adrenaline, accelerating the use of adenosine triphosphate cells, promotes its metabolism and elevated levels of adenosine monophosphate and adenosine (AD). Tripeptide glutathione occupies a special place among the biotions that form its oxidation-reduction system. For more adequate use of adenosine and its analogues in clinical practice, a more detailed comparative study of the effect of adrenaline, adenosine and its precursor adenosine monophosphate on the antioxidant defense system is needed.
What is the aim of the project?
The aim of the project is to study the effect of Adenosinemonophosphate (AMP), adenosine and sympathetic hyperactivation on the activity of 5'-nucleotidase (5'H), catalase, glutathione reductase (GH) and glutathione peroxidase (GPO) in normal and sympathetic hyperactivation caused by injection adrenaline to experimental animals.
What techniques and methods are used?
Sympathetic hyperactivity (hyperadrenalism) will be created experimentally by intraperitoneal injection of adrenaline at a dose of 0.4 mg per 100 g of body weight. Adenosine monophosphate (AMP) and adenosine (for biochemistry MERCK), injected at 10 μg per day in (total dose of 100 μg. The activity of glutathione reductase (GH) and glutathione peroxidase (GPO) will be determined by the method of S.N. Goncharova. Vlasova with co-author. The activity of 5'-nucleotidase (5'H) will be estimated by the rate of adenosine monophosphate hydrolysis to adenosine and phosphoric acid and will be expressed for micromole H3PO4 per mg protein per minute. Catalase activity will be studied by method of the M.A. Korolyuk and co-author. The research will be carried out based on the biochemical department of the Combined Educational and Scientific Laboratory of the State Medical University of Semey.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Biochemical methods for studying the activity of antioxidant protection enzymes and to conduct research, analyze and interpret the results. At the end of the theoretical course, the student will conduct biochemical research under the guidance of a specialist from the Department of the United Educational and Scientific Laboratory; analyze the results. To analyze the activity of the metabolism enzymes of purine nucleotides and antioxidant defense enzymes with sympathetic hyperactivation, to interpret and formalize the results of studies. Students should learn: 1) Biochemical methods of research (Method of determination of activity of 5'-nucleotidase by Tapbergenov S.O, The activity of adenosine monophosphate reflux (AMP deaminase) and adenosidegminase activity has been reported by S.O. Tapbergenov method) 2) Immunological methods of research (Determination of the size of the leukocytes, Determination of the number of B-lymphocytes, Determination of T-lymphocytes and their subpopulation).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will receive theoretical teaching (lectures about general information of “coronary heart disease” and “activity of the metabolism enzymes of purine nucleotides and antioxidant”).
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Necessary knowledge of biochemistry, normal physiology; - Initial knowledge of the body's regulatory systems, health issues, healthy lifestyles; - To be able to use special scientific and reference literature on the evaluation of antioxidant protection; - Skills of interpreting the data obtained in the course of carrying out scientific research work; - Be able to use the mastered techniques in the future; - Be able to report on the results obtained at a student conference. Subjects passed: Biochemistry.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
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