Projects
Name
[Estado de Mexico] Genes involved in depression, insulin resistance and dyslipidemia during pregnancy.
University
Universidad Autonoma del Estado de Mexico
Domain
Gynaecology/Obstetrics
Departement
Maternal Perinatal Hospital Monica Pretelini Saenz, Laboratory of Genetics, Paseo Tollocan 201 Poniente, Colonia Universidad, Toluca, State of Mexico, Zip Code 50010
Head
Dra. Clara Elisa Ortiz Solalinde
Tutor
Dr. Hugo Mendieta Zeron
Languages
Spanish
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
yes yes yes yes yes yes yes No yes yes yes No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The prevalence of diseases and complications during pregnancy or at the time of birth have been increasing in Mexico and in other parts of the world. Pregnancy and childbirth are critical moments in which the body undergoes many adaptations, some of them, such as psychological changes, are often accompanied by sleep disorders, loss of libido and anxious thoughts. These physical and psychological changes can generate unfavorable effects on the growth and development of the future baby. In addition, the increase of insuline resistance entiles another important problem. The function of this hormone relies on controlling blood glucose levels. A significaltly raise of these leves can affect the ability of ovulation, fertilization and implantation of the embryo leading to abortions. The aforementioned, represent important reasons to delve more into the origin of these disorders, making a particular emphazis on the genetic background of each particular disease.
What is the aim of the project?
To identify and quantify the expression of genes involved in depression, insulin resistance and dyslipidemia during pregnancy.
What techniques and methods are used?
Real-time PCR (Polymerase Chain Reaction) fast and cheap technique used to amplify small segments of DNA (deoxyribonucleic acid) to analyze a part of the genome. For the expression of the genes RNA (ribonucleic acid) will be extracted, while for the search for modifications or gene alterations, DNA will be extracted.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will collaborate with by interviewing patients, taking and collecting blood samples that will be later processed. When enough blood samples are processed, and nucleic acids are extracted with manual kits before using the usual technique for samples which will be real- time PCR, the student will take of this procedure. The student will read and analyze specific results with the help of the tutor and some will be indicated as well as, the realization of statistics about the interviews. Also, the student will have to do literature review of the following topics: diabetes mellitus, gestational diabetes, dyslipidemias, metabolic syndrome and depression, based on the most relevant articles published so far.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes. The student will review articles related to the research and analyze them with the tutor and other people who collaborate in the project, at least 5 article sessions during the month.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The correct use of most common materials used in the laboratory (pipettes and centrifuge) and real- time PCR technic. Basic scientific research skills such as data recollection, critical appraisal, scientific writing.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
Articles
- The adiponectin/leptin ratio is a useful tool to evaluate the metabolic status in an obstetrical intensive care unit. Mendieta Zerón H; Herrera Segura G; Layton Tovar CF; Santillán Benítez JG; Colín Ferreyra Mdel C; Trejo Sánchez DI; Montenegro Morales LP.Rom J Intern Med. 2013 Apr-Jun;51(2):107-13.
- NF-κΒ and SOD expression in preeclamptic placentas. Silva Carmona A; Mendieta Zerón H. Turk J Med Sci. 2016;46(3):783-788.
- NF-κΒ and SOD expression in preeclamptic placentas. Silva Carmona A; Mendieta Zerón H. Turk J Med Sci. 2016;46(3):783-788.
- Influenza A (H1N1) was not associated with obesity in pregnant women living in Toluca; México.Mendieta-Zerón H; Santillán-Benítez JG; Colín-Ferreira Mdel C; Montenegro-Cárdenas A; Núñez-Delira CN; Huitrón- Bravo GG.Rev Salud Publica (Bogota). 2011 Dec;13(6):897-907
- Body Mass Index in Pregnancy Does Not Affect Peroxisome Proliferator-activated Receptor Gamma Promoter Region (-359 to -260) Methylation in the Neonate. Casa madrid E; Amaya Chávez A; Mendieta Zeron H. Ann Med Health Sci Res 2016;6(1):38-43.