Projects
Name
Analysing the ADIPOQ, PRARA, FTO, ACE, GHRL gene variants correlation with obesity in Turkish Cypriot population
University
Northern Cyprus (MSANC) - Near East University, Nicosia
Domain
Genetics
Departement
Department of Medical Biology
Head
Assoc. Prof. Dr. Mahmut Çerkez Ergören
Tutor
Assoc. Prof. Dr. Mahmut Çerkez Ergören
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Obesity is a worldwide public health problem. Genetic factors and environment-gene interaction play a 40-70% role in the development of obesity. Many research have been conducted for the identification of candidate genes causing and associated with obesity. According to these research, Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ), Ghrelin and Obestatin Prepropeptide (GHRL), angiotensin-converting enzyme (ACE), angiotensinogen (AGT) and fat-mass and obesity-related (FTO) are the key genetic factors associated with obesity. To date, for Turkish Cypriot population no association studies have been conducted with obesity and above mentioned gene variants.
What is the aim of the project?
The aim of this project is to investigate the relationship between obesity (a metabolic disorder which it’s aetiology is not fully established), obesity-related genes and gene variations. Our aim in advanced plans are to use these in diagnosis by the clinicians, and to help the nutritionist to develop diet programs according to the genetic structure of the person. Secondly, our goal is to investigate distribution of gene polymorphisms that cause obesity in the Turkish Cypriot population.
What techniques and methods are used?
DNA isolation will be performed from the blood samples which arrives to Molecular Diagnostic Laboratory- Research Center of Experimental Health Sciences. For this process EZ1 DNA extractor (QIAGEN) will be used. Concentration measurements of DNA extractions obtained from the samples will be performed by nano-drop spectrophotometer (ACTGene) present in the Medical Genetics Laboratory of the Near East University. In order to carry out genotypic analysis of 11 rationally selected variants of 6 genes involved in vitamin D process, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and real-time polymerase chain reaction methods will be used.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
. Students should be able to handle blood samples according to safe and healthy measures which arrives to Molecular Diagnostic Laboratory- Research Center of Experimental Health Sciences. . Perform DNA extraction from the blood samples. . Measure DNA extractions obtained from the samples performed with nano-drop spectrophotometer (ACTGene) present in the Medical Genetics Laboratory of the Near East University. . Following this process, students should be able to perform polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and real-time polymerase chain reaction methods.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Before the practical sessions, necessary preliminary readings will be supplied by the supervisor and the students are responsible for reading them before the sessions. All details required to perform and understand the methods in the practicals will be explained by the supervisor Assoc. Prof. Dr. Mahmut Cerkez Ergoren.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Students should be able to work under Healthy and Safety measures of a laboratory. All necessary methods and techniques will be explained and taught by the supervisor. Students should have passed the following subjects: Medical Biology and Genetics
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
Articles
- YC; Weisnagel SJ; Argyropoulos G; Walts B; Perusse L; Bouchard C: The human obesity gene map: The 2005 update. Obesity (Silver Spring) 2006; 14: 529–644.
- Maes HH; Neale MC; Eaves LJ (1997) Genetic and environmental factors in relative body weight and human adiposity. Behav Genet 27(4):325–351
- Pérusse L; Bouchard C (2000) Gene–diet interactions in obesity. Am J Clin Nutr 72(5 Suppl):1285S–1290S
- Bell CG; Walley AJ; Froguel P (2005) The genetics of human obesity. Nat Rev Genet 6(3):221–234
- BienertovaVasku J; Bienert P; Sablikova L; Slovackova L; Forejt M; Piskackova Z; Kucerova L; Heczkova K; Brazdova Z; Vasku A (2009) Effect of ID ACE gene polymorphism on dietary composition and obesity-related anthropometric parameters in the Czech adult population.Nutr 4(30):207–213.
- Van den Bree MB; Eaves LJ; Dwyer JT (1999) Genetic and environmental influences on eating patterns of twins aged >50 year. Am J Clin Nutr 70(4):456–465
- Maes HH; Neale MC; Eaves LJ (1997) Genetic and environmental factors in relative body weight and human adiposity. Behav Genet 27(4):325–351
- Pérusse L; Bouchard C (2000) Gene–diet interactions in obesity. Am J Clin Nutr 72(5 Suppl):1285S–1290S
- Kourlaba G; Pitsiladis YP; Lagou V; Grammatikaki E; Moran CN; Kondaki K; Roma-Giannikou E; Manios Y (2008) Interaction effects between total energy and macronutrient intakes and angiotensin-converting enzyme 1 (ACE) I/D polymorphism on adiposity-related phenotypes in toddlers and preschoolers: the growth; exercise and nutrition epidemiological study in preschoolers: the GENESIS study. Br J Nutr 100(6):1333–1340