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Lipid dynamics in Vascular Dementia model observed by Desorption electrospray ionization mass spectrometry imaging
Federal University of Goiania (UFG)
Department of Pharmacology, Institute of Biological Sciences, Federal University of Goias. Esperança St., S/N. Samambaia Campus, ICB II Building, Room 114. CEP 74690-900, Goiânia - Goiás - Brazil.
Prof. Dr. Mauro Cunha Xavier Pinto
Prof. Dr. Mauro Cunha Xavier Pinto Dra. Onésia Cristina Oliveira Lima
English and Portuguese
Type of Research Project
- Basic science
What is the background of the project?
Vascular dementia is caused by problems in cerebral blood supply and it promotes deep changes in brain lipid homeostasis. Understanding the expression pattern of these lipids in the brain may reveal biomarkers and novel pathways for the treatment of the disease. Desorption electrospray ionization mass spectrometry (DESI-MS) is an imaging technique used to directly analyses spatial distribution of molecules from biological samples. The technique is based in the extracted molecules from the sample surface by a small spray of charged droplets, their analyses in mass spectrometer and the two dimensional map of the molecules in the surface. The DESI imaging of brain samples can be applied to reveal several neurochemical signatures of pathologies, such Parkinson’s disease, Stroke, Alzheimer’s disease, Vascular Dementia and others. In addition, it can be used to identify biomarkers of diseases and new pathological pathways.
What is the aim of the project?
Performing the two vessel occlusion (2VO) model for Vascular Dementia and using the Desorption Electrospray Ionization - Imaging Mass Spectrometry (DESI-IMS) technique for imaging of lipids on rat brain tissues in order to seek for biomarkers. In this work, we will map the lipids and investigated the brain chemical profile of the rat brain subjected to cerebral blood flow reduction.
What techniques and methods are used?
Surgical techniques (Two vessel occlusion model-2VO and Middle Cerebral Artery Occlusion - MCAO - MCAO); Behavioral analyzes (open field test, plus maze, Y-maze, Novel object recognition test and morris water maze). Euthanasia of animals and tissue collection; Preparation of solutions; Preparations of histological sections; Desorption Electrospray Ionization - Imaging Mass Spectrometry analysis. Multivariate Data Analysis Methods: Partial least squares discriminant analysis (PLS‐DA); Principal Component Analysis (PCA).
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Research laboratory training, personal protective equipment and biosafety. Weighing of materials, preparation of solutions, homogenization of solutions, pH measurement and pipetting techniques. Management of rodents (rats and mice), rodent immobilization, rodent solutions injection and rodent euthanasia. After animal management training, the student will learn and train the 2VO and MCAO surgeries on rodents, and then, extract and dissect the brain. The DESI-MS analyzes will be performed by trained professionals and the student will have the opportunity to observe. The student will perform the statistical analysis of PCA and PLS-DA.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be theoretical lectures for the student. The Neurolab deliver for academic community weekly seminars and lectures about neuroscience and neurochemistry.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Willingness and ability to work with animals and biological samples.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Glycine transporters type 1 inhibitor promotes brain preconditioning against NMDA-induced excitotoxicity. Pinto MC; Lima IV; da Costa FL; Rosa DV; Mendes-Goulart VA; Resende RR; Romano-Silva MA; de Oliveira AC; Gomez MV; Gomez RS. Neuropharmacology. 2015 Feb;89:274-81.
- Neurochemical study of amino acids in rodent brain structures using an improved gas chromatography-mass spectrometry method. Pinto MC; de Paiva MJ; Oliveira-Lima OC; Menezes HC; Cardeal Zde L; Gomez MV; Resende RR; Gomez RS. J Chem Neuroanat. 2014 Jan;55:24-37.
- Pharmacological induction of ischemic tolerance in hippocampal slices by sarcosine preconditioning. Pinto MC; Mourão FA; Binda NS; Leite HR; Gomez MV; Massensini AR; Gomez RS. Neurochem Int. 2012 Oct;61(5):713-20.
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