Projects
Name
Molecular Point of Care for Rapid, Portable and Easy Diagnosis of Pediatric Infectious Diseases
University
Catalonia (AECS) - Catalan International University (UIC), Barcelona
Domain
Microbiology
Departement
Molecular Microbiology Department
Head
Carmen Muñoz-Almagro
Tutor
Carmen Muñoz-Almagro
Languages
English and intermediate Spanish required
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No Yes No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Invasive infectious diseases demand rapid etiological diagnosis of the causative pathogen to orientate early and adequate treatment and save lives, especially in the vulnerable paediatric population. Bacteria, viruses, parasites and fungi are able to invade natural sterile body sites, causing potentially life-threatening and frequently under-diagnosed diseases, such as sepsis, meningitis, or encephalitis. Current microbiological methods have important limitations in capability to detect unsuspected pathogens, diagnostic accuracy, and time to result. There is a need for new tests allowing fast, accurate, unbiased identification of pathogens in direct clinical samples. Their introduction could expand and improve microbiological diagnosis of invasive diseases, facilitate earlier switch to more targeted anti-microbial therapies, and enhance epidemiological surveillance.Recent tecnological advances have potential to pave the way towards the new rapid, easy and portable diagnosis of infections.
What is the aim of the project?
To evaluate an easy and portable molecular point of care to rapid diagnosis of pediatric infectious diseases.
What techniques and methods are used?
A rapid and portable device for rapid diagnosis of a sindromic pediatric infectious disease (i.e respiratory infections, gastrointestinal infections or meningoencephalitis infections will be evaluate for the student. 5 steps will be needed: 1) literature studies, 2) lab work 3) collecting data, 4) scientific writing and report 5) Ethical aspects related to the research activity.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
-Literature review - Identification, registration and management of diagnostic samples - Management of molecular point of care device - Registration of results and study variables in datasheet - Statistical analyisis - Draft of report All this task will be performed under supervision and training. For training the student will participate as observer in the routine activities of the molecular microbiology department including clinical sessions with pediatrician and lab work with tecnitians.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes. The student will be advised for some preliminary readings and he/she have to attend to the diary sessions of the department.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
MS office (power point, word, excel) Desirable: previous training in lab work and biostatistical. Subjects passed: Microbiology. Certificate of: Criminal record.
Are there any legal limitations in the student’s involvement
Yes
The laboratory where the project is located is in a pediatric hospital. For this reason, the student need to show us his criminal record. The logbook will be revised by the professor at the end of the exchange. All the document of the students have to be ready 3 months before the internship starts, specially the criminal record.
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
- Evaluation of the eazyplex MRSA assay for the rapid detection of Staphylococcus aureus in pleural and synovial fluid. Henares D; Brotons P; Buyse X; Latorre I; de Paz HD; Muñoz-Almagro C. Int J Infect Dis. 2017 Jun;59:65-68. doi: 10.1016/j.ijid.2017.04.013. Epub 2017 Apr 24
- Validation of a loop-mediated isothermal amplification assay for rapid diagnosis of pertussis infection in nasopharyngeal samples. Brotons P; de Paz HD; Esteva C; Latorre I; Muñoz-Almagro C. Expert Rev Mol Diagn. 2016;16(1):125-30. doi: 10.1586/14737159.2016.1112741. Epub 2015 Nov 13.
- Infectious cause of death determination using minimally invasive autopsies in developing countries. Martínez MJ; Massora S; Mandomando I; Ussene E; Jordao D; Lovane L; Muñoz-Almagro C; Castillo P; Mayor A; Rodriguez C; Lopez-Villanueva M; Ismail MR; Carrilho C; Lorenzoni C; Lacerda MVG; Bassat Q; Menéndez C; Ordi J; Vila J. Diagn Microbiol Infect Dis. 2016 Jan;84(1):80-86. doi: 10.1016/j.diagmicrobio.2015.10.002. Epub 2015 Oct 9.
- Design and validation of a real-time RT-PCR for the simultaneous detection of enteroviruses and parechoviruses in clinical samples. Cabrerizo M; Calvo C; Rabella N; Muñoz-Almagro C; del Amo E; Pérez-Ruiz M; Sanbonmatsu-Gámez S; Moreno-Docón A; Otero A; Trallero G; study group of Enterovirus and parechovirus infections in children under 3 years-old; Spain (PI12-00904). J Virol Methods. 2014 Nov;208:125-8. doi: 10.1016/j.jviromet.2014.08.008. Epub 2014 Aug 22.
- Validity of a minimally invasive autopsy for cause of death determination in stillborn babies and neonates in Mozambique: An observational study. Menendez C; Castillo P; Martínez MJ; Jordao D; Lovane L; Ismail MR; Carrilho C; Lorenzoni C; Fernandes F; Nhampossa T; Hurtado JC; Navarro M; Casas I; Santos Ritchie P; Bandeira S; Mocumbi S; Jaze Z; Mabota F; Munguambe K; Maixenchs M; Sanz A; Mandomando I; Nadal A; Goncé A; Muñoz-Almagro C; Quintó L; Vila J; Macete E; Alonso P; Ordi J; Bassat Q. PLoS Med. 2017 Jun 20;14(6):e1002318. doi: 10.1371/journal.pmed.1002318. eCollection 2017 Jun.