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Development of Adenoviral-Based Oncolytic Virus Therapy
Japan (IFMSA-Japan) - Kagoshima University, Kagoshima
Department of Gene Therapy and Regenerative Medicine Kagoshima University Graduate School of Medical and Dental Sciences 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan
Ken-ichiro Kosai, M.D., Ph.D.
Nobuhiro Ijichi, Ph.D., Kaoru Mitsui, Ph.D.
Required: English/Japanese Accepted: English/Japanese
Type of Research Project
- Basic science
What is the background of the project?
We developed a method to efficiently construct diverse Conditionally replicating adenoviruses (CRAs), also called oncolytic adenoviruses, that can specifically target and/or efficiently treat malignant tumors using multiple factors (m-CRAs). Our m-CRA construction system expedited the process of generating, modifying, and testing diverse m-CRAs with the goal of developing an ideal m-CRA for tumor therapy; indeed, our m-CRA strategy increased the potential cancer specificity of virotherapy.
What is the aim of the project?
To develop adenovirus-based oncolytic vectors with high specificity to cancer cells and increased tumor killing efficiency.
What techniques and methods are used?
Basic molecular biology assays (PCR, recombinant DNA technology), Cell culture, Gene transduction (Plasmid DNA, Adenovirus vector, Retrovirus vector), Flowcytometry, etc
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The students are expected to participate the laboratory experiments and undergo training in laboratory safety practice. The students will learn molecular and virology techniques including: Cloning (recombinant DNA technology), DNA/RNA preparation/extraction/purification, Gene transduction, Western blot, Flowcytometry, Cell culture, Virus production/assay. Students will be led by senior members of the laboratory and are expected to have hands-on participation.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings and short lectures will be provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of biochemistry and molecular biology.
Are there any legal limitations in the student’s involvement
Students may be required to sign a confidentiality agreement regarding the data they learned from participating in the project.
Type of students accepted
This project accepts: - Medical students
- Mitsui K; Ide K; Takayama A; Wada T; Irie R; Kosai K.(2015) Conditionally replicating adenovirus prevents pluripotent stem cell-derived teratoma by specifically eliminating undifferentiated cells. Mol Ther Methods Clin Dev. Aug 12;2:15026
- Tanoue K; Wang Y; Ikeda M; Mitsui K; Irie R; Setoguchi T; Komiya S; Natsugoe S; Kosai K (2014) Survivin- responsive conditionally replicating adenovirus kills rhabdomyosarcoma stem cells more efficiently than their progeny. J Transl Med. 12; 27.
- Horikawa Y; Wang Y; Nagano S; Kamizono J; Ikeda M; Komiya S; Kosai KI (2011) Assessment of an altered E1B promoter on the specificity and potency of triple-regulated conditionally replicating adenoviruses: implications for the generation of ideal m-CRAs. Cancer Gene Ther.; 18(10):724-33.
- Kamizono J; Nagano S; Murofushi Y; Komiya S; Fujiwara H; Matsuishi T; Kosai K (2005) Survivin- responsive conditionally replicating adenovirus exhibits cancer-specific and efficient viral replication. Cancer Res. 2005; 65(12):5284-91.
- Nagano S; Oshika H; Fujiwara H; Komiya S; Kosai K (2005) An efficient construction of conditionally replicating adenoviruses that target tumor cells with multiple factors. Gene Ther. 2005; 12(18):1385-93.
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