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Viral Vector-Based Approaches for Safer Regenerative Medicine.
Japan (IFMSA-Japan) - Kagoshima University, Kagoshima
Department of Gene Therapy and Regenerative Medicine Kagoshima University Graduate School of Medical and Dental Sciences 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan
Ken-ichiro Kosai, M.D., Ph.D.
Kaoru Mitsui, Ph.D., Nobuhiro Ijichi, Ph.D.
Required: English/Japanese Accepted: English/Japanese
Type of Research Project
- Basic science
What is the background of the project?
Human pluripotent stem cells (hPSCs) are a promising source of regenerative material for clinical applications. However, hPSC transplant therapies pose the risk of teratoma formation and malignant transformation of undifferentiated remnants. We have recently embarked on studies aimed at locating and directly treating hPSC- derived tumorigenic cells.
What is the aim of the project?
To develop novel approaches to directly killing tumorigenic cells by transduction of suicide genes and oncolytic viruses for the safety of hPSC-based therapy.
What techniques and methods are used?
Basic molecular biology assays (PCR, recombinant DNA technology), Cell culture, Gene transduction (Plasmid DNA, Adenovirus vector, Retrovirus vector), Flowcytometry, etc
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The students are expected to participate the laboratory experiments and undergo training in laboratory safety practice. The students will learn molecular and virology techniques including: Cloning (recombinant DNA technology), DNA/RNA preparation/extraction/purification, Gene transduction, Western blot, Flowcytometry, Cell culture, Virus production/assay. Students will be led by senior members of the laboratory and are expected to have hands-on participation.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings and short lectures will be provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of biochemistry and molecular biology.
Are there any legal limitations in the student’s involvement
Students may be required to sign a confidentiality agreement regarding the data they learned from participating in the project.
Type of students accepted
This project accepts: - Medical students
- Ide K; Mitsui K; Irie R; Matsushita Y; Ijichi N; Toyodome S; Kosai K. (2017) A Novel construction of lentiviral vectors for eliminating tumorigenic cells from pluripotent stem cells. Stem Cells. DOI: 10.1002/stem.2725
- Mitsui K; Ide K; Takahashi T; Kosai K. (2017) Viral Vector-Based Innovative Approaches to Directly Abolishing Tumorigenic Pluripotent Stem Cells for Safer Regenerative Medicine. Mol Ther Methods Clin Dev. 5:51-58 (Review)
- Sakamoto K; Khai NC; Wang Y; Irie R; Takamatsu H; Matsufuji H; Kosai KI. Heparin-binding epidermal growth factor-like growth factor and hepatocyte growth factor inhibit cholestatic liver injury in mice through different mechanisms. Int J Mol Med. 2016 10.3892/ijmm.2016.2784
- Mitsui K; Ide K; Takayama A; Wada T; Irie R; Kosai K.(2015) Conditionally replicating adenovirus prevents pluripotent stem cell-derived teratoma by specifically eliminating undifferentiated cells. Mol Ther Methods Clin Dev. Aug 12;2:15026
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