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[Sousse]Genetic susceptibility to autoimmune diseases
Tunisia (ASSOCIA-MED) - University of Sousse/ Faculty of Medecine of Sousse, Sousse
Immunogenetic Unit,Faculty of medicine of Sousse Sousse, Tunisia
Pr.Foued Ben Hadj Slama
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
An autoimmune disease is a condition arising from an abnormal immune response directed against self-components of the body and the consequent immune destruction of host tissues. Human autoimmune diseases affect more than 5% of the population worldwide, and impose a significant burden of morbidity and mortality on the human population. Some common diseases that are generally considered autoimmune include systemic lupus erythematosus, celiac disease, type 1 diabetes, Hashimoto thyroiditis, Graves' disease, rheumatoid arthritis, inflammatory bowel disease and psoriasis. The development of an autoimmune disease is a very complex process in which certain environmental influences, such as cigarette smoke or infectious agents may interplay with genetic factors to initiate the disease. Autoimmune diseases are inherited as a complex trait, with multiple loci controlling various aspects of disease susceptibility.
What is the aim of the project?
We aimed to investigate whether putative or proven functional variants of genes encoding proteins that are key components of the immune system are associated with particular autoimmune diseases.
What techniques and methods are used?
- Using case-control study design a technique which is designed to help determine if an exposure is associated with an outcome, So we can investigate type 1 diabetes, systemic lupus erythematosus or rheumatoid arthritis patients. - Patients and control subjects are recruited. - One blood sample is collected for each Subject. - All the samples are processed for DNA extraction which is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. - the extraction of DNA generally follows three basic steps: 1. Lyse (break open) the cells. 2. Separate the DNA from the other cell components. 3. Isolate the DNA. - Specific regions of DNA ARE amplified by Polymerase chain reaction (PCR) which is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. - Following amplification, PCR products are electrophoresed on agarose gels. - Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. - Genotypes are determined in the different groups and compared using statistical tests.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
- The student will be responsible to manage samples centrifugation to be evaluated - The student will contribute on : • Serum preparation : Collect whole blood in a covered test tube. • Plasma preparation : Collect whole blood into commercially available anticoagulant-treated tubes - The student will prepare materiel and products for PCR and electrophoresis - He will participate on genotypes counting - the student will be expected to perform data analysis and assay validation. - Statistical analysis of results and a brief report discussing it shall be prepared by the student until the end of the clerkship.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- - Setia MS. Methodology Series Module 2: Case-control Studies. Indian Journal of Dermatology. 2016;61(2):146-151. doi:10.4103/0019-5154.177773.
- - Single nucleotide polymorphisms (SNPs) and Restriction Fragment Length Polymorphisms (RFLPs) http://medschool.slu.edu/gpbs/uploads/2008/Lecture%206.pdf
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