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Role of immune system in glaucoma
Germany (bvmd) - Ruhr-Universitaet, Bochum
Experimental Eye Research Institute, University Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892 Bochum, Germany
PD Dr Stephanie Joachim
PD Dr Stephanie Joachim
Type of Research Project
- Basic science
What is the background of the project?
The project is based on a well-established glaucoma animal model, which is used to induce retinal damage, like retinal ganglion cell death and axonal degeneration. Subsequent to these in vivo studies and within the framework of the project the extend of retina and optic nerve damage should be analyzed and the underlying mechanisms like the contribution of immune cells, cell death mechanisms and so on should be identified. In addition, novel biomarkers for glaucoma will be investigated.
What is the aim of the project?
Investigate glaucoma pathomechanisams and detect novel biomarker for this disease.
What techniques and methods are used?
In this project mainly immunohistological stainings are performed. Immunohistochemistry involves the process of selectively imaging antigens in cells of a tissue section. In this project retina and optic nerve sections will be analyzed. In addition, the polymerase chain reaction technique (qRT-PCR) will used. It is a common laboratory technique used to make many copies of a region of DNA. These evaluations are followed by statistical analysis.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students can work with retinal organ cultures or with animal models for eye diseases (under supervision). In vivo examinations include measurement of intraocular pressure or retinal function (ERG). Afterwards, they are carrying out different immunohistological stainings and polymerase chain reaction technique (qRT-PCRs) of ocular tissues. In addition, the evaluation of all results is performed by the student. These includes e.g. cell counts and subsequent statistical analysis. Besides, all results should be appropriately presented and related to the literature.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Once a week, a laboratory seminar will be held.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
To be able to work independently, sense of responsibility, and motivation. Possibly basic knowledge of ophthalmoloy and laboratory work.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system - Students in biomedical fields
- Casola; C; Schiwek; JE; Reinehr; S; Kuehn; S; Grus; FH; Kramer; M; Dick; HB and Joachim; SC (2015). "S100 Alone Has the Same Destructive Effect on Retinal Ganglion Cells as in Combination with HSP 27 in an Autoimmune Glaucoma Model." J Mol Neurosci 56(1): 228-236.
- Joachim; SC; Gramlich; OW; Laspas; P; Schmid; H; Beck; S; von Pein; HD; Dick; HB; Pfeiffer; N and Grus; FH (2012). "Retinal ganglion cell loss is accompanied by antibody depositions and increased levels of microglia after immunization with retinal antigens." Plos One 7(7): e40616.
- Laspas; P; Gramlich; OW; Muller; HD; Cuny; CS; Gottschling; PF; Pfeiffer; N; Dick; HB; Joachim; SC and Grus; FH (2011). "Autoreactive antibodies and loss of retinal ganglion cells in rats induced by immunization with ocular antigens." Invest Ophthalmol Vis Sci 52(12): 8835-8848.
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