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Nanomedicine and Immunology: How to study T cell responses
Universita degli studi di Firenze
Experimental and Clinical Medicine
Prof. Corrado Poggesi
Prof. Mario Milco D’Elios
Type of Research Project
- Basic science
What is the background of the project?
Set-up novel approach to immunology via nanotechnologies. Lots is known upon the importance of T cells in host responses. For example the 2018 Nobel Prize in Medicine has been given to two immunologists for their studies on the relevance of CTLA-4 and PD1 in shaping T-cell responses. The knowledge of T cell functions has allowed to treat with immunotherapy cancer and transplant rejection. In response to different microorganisms. Viruses can be successfully eliminated only by killing their host cells by CD8+ class I MHC restricted cytotoxic T lymphocytes, which recognize viral antigens synthesized within infected cells and presented on their surface in the context of class I MHC molecules. In contrast, most of microbial antigens are endocytosed by antigen-presenting cells, processed and presented preferentially in association with MHC class II molecules to CD4+ class II MHC-restricted T helper cells.
What is the aim of the project?
Aim of the study is to obtain novel tools to be used for diagnosis and therapy using T-cell responses to evaluate the personalized immune responses to different antigens and thus to evaluate in this way the “healthy” of the immune system of the person under study.
What techniques and methods are used?
Learning of short-term and long-term cell culture techniques as well as other nano-immunological assays (enzyme-linked immunosorbent assay - ELISA, Enzyme-Linked ImmunoSpot - ELISpot, proliferation assays). T cells will be cultured with different antigens (e.g. Helicobacter pylori, Mycobacterium tuberculosis, Chlamydia pneumoniae) or mitogens (e.g. Phytohaemagglutinin, Phorbol myristate acetate, ionomycin) for 5 days, then keep in cultured with human recombinant interleukin 2. And every fifteen days re-stimulated with antigen or PHA and irradiated peripheral blood mononuclear cells, Then the T cells will be tested for cytokine secretion
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
To acquire the basis of nanoimmunology. Get familiar with the basic nanoimmunologic techniques. The student will work in a settings of immunologic laboratory. The student will work under a flow laminar bench and will used microscopes, cytofluorimeter, ELISA and ELISPOT automated readers. The students is expected to learn the basis of investigating T cell functions, such as proliferation, cytokine production.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary knowledge of the physiology of T cells and B cell functions. During the stage the students will participate to seminars, lectures, readings related to immunology and nanotechnology.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
General knowledge of the immune system
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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