Laboratory diagnostic of active and latent tuberculosis.
Bosnia and Hrezegovina (BoHeMSA) - Univerity of Tuzla, Tuzla
Microbiology Department, University Clinical Center Tuzla,
Prof. dr. Nijaz Tihić
Prof. dr. NijazTihić
English, Bosnian, Croatian, Serbian
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Tuberculosis is one of the leading diseases in the world. Bosnia and Herzegovina belongs to country with medium prevalence and incidence of tuberclosis. It is very important to diagnose tuberculosis as soon as possible. Different methods are used in routine diagnostic using traditional (microsopy and culture techniques) and molecular methods (PCR- „Polymerase chain reaction“ technique). Patients in contact with Mycobacterium tuberculosis can develop latent and active infection. It is very important to determine the status of infection in order to start with appropriate treatment and preventive measure.
What is the aim of the project?
To analyze laboratory diagnostic methods used for diagnosis of active and latent tuberculosis, to determine prevalence and inicdence of active and latent tuberculosis in Tuzla Canton and to determine profile of drug sensitivity isolates on first line drugs.
What techniques and methods are used?
Candidate will learn and perform different laboratory techniques important for diagnostic tuberculosis: microscopy examination, culture technique on solid and liquid media, performing drug susceptibility test, phneotypic and molecular identification test, perform and data interpretation of Iinterferon gama realease assay; perform statistical analysis data using SPSS program for Windows, presentation results.
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Student is expected to search relevant literature for the project, to practice critical thinking about different methods and techniques used for laboratory diagnostic tuberculosis, to be able to use and analyze laboratory data, to perform basic statistical analysis. Student is expected to examine microscopy slides, to process respiratory specimens for cultivation on solid and liquid media. Student will be able to recognize colony morphology different Mycobacteria species and to perform drug susceptibility testing on liquid media and molecular identification Mycobacteria using PCR(Polymerase chain reaction)/Hybridization method. Student is expected to understand principle of interferfongama release assay and its use for diagnostic latent tuberculosis and perform Quantiferon test.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Introduction seminar about the work process, what we already did and what we will do.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a presentation
- The student will prepare a scientific report
- The student will prepare an abstract
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- S.A. Watterson; F.A. Drobniewski. Modern laboratory diagnosis of mycobacterial infections. J CinPathol 2000;53:727-732
- Susan E. Dorman. New Diagnostic Tests for Tuberculosis: Bench; Bedside and Beyond. Clinical Infectious Diseases 2010;50(S3):S173-S177