Role of the lysophospholipid mediators in lung fibrosis.
Japan (IFMSA-Japan) - Kawasaki Medical University, Okayama(Kansai area)
Department of Pharmacology, Kawasaki Medical School, 577 Matsushima, Kurashiki, 701-0192, Japan
Yasuo Okamoto
Yasuo Okamoto, Kazuhito Tsuboi, Yasuhiro Takenouchi
Required: English Accepted: German, French, Italian, Spanish, Dutch
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No Yes Yes Yes Yes Yes Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are pleiotropic lysophospholipid mediators and regulate various biological processes via G protein–coupled receptors. In addition, several enzymes that utilize LPA/S1P as a substrate or generate it as a product are under its regulatory control. Recent investigations indicate that LPA/S1P may play a critical role in regulating the development of lung fibrosis. Increased LPA/S1P has been found in bronchoalveolar lavage fluid (BALF) in lung fibrosis after bleomycin-induced lung injury. Therefore, it is necessary to understand how they regulate the metabolism of LPA/S1P in lung fibrosis. Lipid phosphate phosphatases (LPPs) play an important role in regulating LPA/S1P signaling through the degradation of these bioactive lipids. Recently we found that mRNA expression of LPPs was decreased in bleomycin-induced lung fibrosis, suggesting that this is associated with increased LPA/S1P levels in BALF.
What is the aim of the project?
The aim of the project is to investigate the involvement of lipid phosphate phosphatases (LPPs) in the pathogenesis of lung fibrosis.
What techniques and methods are used?
 Culturing cell lines  Animal model  Extracting RNA, and proteins from cell lines and tissues.  Polymerase chain reaction (PCR), Western blotting, Enzyme-Linked ImmunoSorbent Assay (ELISA), Enzyme assay, Determination of protein concentration.  Immunostaining of tissues and observation using bright-field microscope and fluorescent microscope.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Biochemical and molecular biology techniques will be performed by the students under supervision, for example, cell culture, polymerase chain reaction (PCR), western blotting, immunofluorescence, enzyme assay. The students will also learn how to analyze and evaluate their own experimental data. The student will attend weekly Department's meeting on Research in Progress, where they are expected to present their experimental data as well as future experimental plans that are evaluated and discussed with faculty.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings and short lectures will be provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
 Reading and understanding scientific reports.  Handling basic laboratory devices such as pipettes, micropipettes, test tubes, and dishes.  Handling animals.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- 1. Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6. Ohkura SI; Usui S; Takashima SI; Takuwa N; Yoshioka K; Okamoto Y; et. al.; Plos One. 12:e0182329; 2017
- 2. Sphingosine-1-phosphate as a mediator involved in development of fibrotic diseases. Takuwa Y; Ikeda H; Okamoto Y; Takuwa N; Yoshioka K. BBA.-Mol. Cell Biol. L. 1831:185-192; 2013