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Zika virus vaccines: evaluation of humoral and cellular responses induced by Zika virus vaccines prepared with different adjuvants.
Federal University of Rio Grande do Sul (UFRGS)
Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciencias Basicas da Saude (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS) Av. Sarmento Leite 500, Porto Alegre - RS, Centro Historico, CEP 90050-170
Profa. Mercedes PassosGeimba
Prof Paulo Michel Roehe
English, Spanish, Portuguese
Type of Research Project
- Basic science
What is the background of the project?
Immunization with vaccines or pre-exposure to different arboviruses may either induce crossprotection or enhance deleterious effects of infection in the host. Cross protection is suggested by the fact that the cases of microcephaly associated to Zika Virus infections were detected essentially in an area which is not covered by yellow fever vaccination in the country. On the other hand the “antibody mediated enhancement” of infection is a recognized phenomenon which has already been demonstrated to occur in vitro with yellow fever virus and Zika virus. The present project will be carried out to quantitate the effect of immunizations with different vaccines to arboviruses and to compare theeffect of natural saponine-based adjuvants on vaccines to prevent infections with relevant arboviruses (Zika, Yellow Fever, Dengue and Chikungunya viruses). using mice as experimental models.
What is the aim of the project?
To evaluate the effect of natural saponin-based adjuvants and, eventually, to develop adequate Zika virus vaccine formulations for human protection.
What techniques and methods are used?
Work on cell cultures; virus multiplication and titration in plaque assays and other quantitative assays; experimental vaccine production and formulation, nucleic acid detection assays for Zika and other flaviviruses, different enzyme immunoassays for detection of antibodies and citokines; immunoperoxidase in cell culture and plaque reduction neutralization assays;
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be expected to participate in all aspects of laboratory work, including cell culture and virus multiplication and quantitation, assays aiming nucleic acid detection (e.g. Polymerase chain reaction - PCR), enzyme immunoassays (Enzyme-Linked Immunosorbent Assay - ELISA), and flow cytometry. Beware that the project involves experimental animal inoculations, so although all measures are taken to avoid all possible animal discomfort, a substantial part of the project involves animal handling, so the applicant must be prepared to accompany such activities.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Periodical meetings for discussion of the work flow and seminars on the subjects covered by the project will be part of the activities of the students.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Knowledge on basic virology; commitment to learning and familiarity with experimental animal work.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- SCHULER-FACCINI; LAVÍNIA ;Roehe; Paulo ; ZIMMER; EDUARDO R. ; QUINCOZES-SANTOS; ANDRÉ ; DE ASSIS; ADRIANO M. ; LIMA; ELIZABETH OBINO CIRNE ; GUIMARÃES; JORGE ALMEIDA ; VICTORA; CESAR ; NETO; VIVALDO MOURA ; SOUZA; DIOGO O. . ZIKA Virus and Neuroscience: the Need for a Translational Collaboration. Molecular Neurobiology; v. 2017; p. 1-5; 2017.
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