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Environmental health and bacterial control analysis
Spain (IFMSA Spain) - University of Granada, Granada
Department of Preventive Medicine and Public Health.
Miguel García Martín
Elena Espigares Rodriguez
Type of Research Project
- Basic science
What is the background of the project?
Isolation and identification of bacterial strains (Enterobacteriaceae and Staphylococci) and study of sensibility of antibiotics and resistance genes. Nasal swab samples were collected during 2016 from farm animals (bovine and lamb), exposed personnel who visit the farm on daily basis such as ranchers, veterinarians, students and lecturers from the university that take practices on the installation, and non-exposed personnel such as first year students who do not have practice classes on farm (Université des Frères Mentouri (Constantine) and Institut des Sciences El Khroub (Algeria).
What is the aim of the project?
Study of different sources of infection of bacteria of sanitary interest. Study of carriers of Staphylococcus aureus. We will conclude the prevalence of S. aureus carriers in the population and the resistance of isolated strains to antibiotics.
What techniques and methods are used?
- Bacterial cultures (Mannitol salt Agar, Brain Heart Infussion Agar, MacConkey Agar). - Kirby-Bauer antimicrobial disk diffusion - PCR (Polymerase chain reaction)
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Lab work: Bacterial cultures (Mannitol salt Agar, Brain Heart Infussion Agar, MacConkey Agar….); Biochemical tests to identify bacteria (coagulase test…); Kirby-Bauer antimicrobial disk diffusion (determine the susceptibility of differents bacterial species to various antibiotics); Polymerase Chain Reaction or PCR (based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings: Protocol laboratory techniques and previously published papers in relation to this topic by our team.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Previous laboratory experience
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Phenotypic Resistance to Disinfectants and Antibiotics in Methicillin-Resistant Staphylococcus aureus Strains Isolated from Pigs (2016). E. Espigares; E. Moreno Roldan; M. Espigares; R. Abreu; B. Castro; A. L. Dib and_A. Arias. Zoonoses and Public Health. 64 (4): 272-280.
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