Analysis of function of inflammatory cells and cancer cells.
Japan (IFMSA-Japan) - Kawasaki Medical University, Okayama(Kansai area)
Department of Biochemistry, Kawasaki Medical School 577 Matsushima, Kurashiki, 701-0192, Japan
Prof Futoshi Kuribayashi
Prof Akira Yamauchi
Required: English Accepted: German, French, Italian, Spanish, Dutch
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes No No No No Yes No Yes Yes Yes Yes
Type of Research Project
- Basic science
What is the background of the project?
Inflammatory cells, such as neutrophils, macrophages, and lymphocytes, play an important role in host defense and migrate toward a focus of inflammation. This directional migration is altered by various kinds of cytokines. Cancer cells also migrate inside tissues, which cause metastasis and invasion. The cancer cell migration is also altered by various kinds of molecules within tissues. To clarify the mechanism how these cells are activated or regulated by the microenvironment is very important for caring inflammatory diseases and cancer.
What is the aim of the project?
Analysis of interaction among inflammatory cells, cancer cells and surrounding tissues, and to find the mechanisms of regulating the function of these cells.
What techniques and methods are used?
Culturing cell lines. Extracting DNA, RNA, and proteins from cell lines. Polymerase chain reaction (PCR), Western blotting, Flow-cytometry, Enzyme-Linked ImmunoSorbent Assay (ELISA), Phosphorylation Antibody Array, and Cell migration assay. Immunostaining of tissues and observation using bright-field microscope and fluorescent microscope.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The tasks include common techniques used in biology such as Polymerase chain reaction (PCR), western blotting, immunofluorescence, transgenic protein expression and gene silencing by si RNA etc. As for the specific experiments, they can perform time-lapse imaging analysis using cell lines. We hope the students to work on the project in a courageous manner.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings and short lectures will be provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Reading and understanding scientific reports. Handling basic laboratory devices such as pipettes, micropipettes, test tubes, and dishes. Also using personal computers.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device. Yamauchi A; et al. BMC cancer. 2017; 17(1):234.