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Epigenetic and non-coding RNA (Ribonucleic acid) regulation during development of the mammalian inner ear: implications for deafness
Israel (FIMS) - Tel Aviv University, Tel Aviv
Department of Human Molecular Genetics & Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Klatchkin St. 35, Tel Aviv
Prof Shimon Efrat
Prof Karen B. Avraham
Type of Research Project
- Basic science
What is the background of the project?
Hearing impairment is one of the most common sensory disabilities, with the majority of known cases caused by genetic mutations that lead to degradation of hair cells. Epigenetic modifications and non-coding Ribonucleic acid (ncRNA) are essential for cell development and maturation, and likely to have a major regulatory role in the development and maturation of the inner ear. Advances in high-throughput sequencing enable us to study ncRNA and epigenetic modifications in the auditory field, while using small amounts of inner ear sensory epithelium or separated cell types as input. We are investigating different levels of regulation, including transcription factors, ncRNA and epigenetic modifications. Those regulations are being examined at three different time points, embryonic day 16.5 (E16.5) post-natal day 0 (P0) and P22, which represent key turning points during the development, differentiation and maturation of mice inner ear sensory epithelium.
What is the aim of the project?
Gain a better understanding of inner ear development and maturation by predicting and validating micro RNA (Ribonucleic acid) targets regulating inner ear expration.
What techniques and methods are used?
Molecular biology techniques including Polymerase chain reaction (PCR), real-time quantitative PCR, expression vector cloning, site-directed mutagenesis, statistical analysis, cloning, sequencing, library construction.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Read manuscript papers provided by the lab, plan experiments with the tutor, learn basic molecular biology techniques including Polymerase chain reaction (PCR), cloning, sequencing, library construction. All the tasks will be taught by the tutor and occasional lectures. The tasks will be performed under the tutor's supervision and with guidance. The student will take part in an ongoing project in the lab alongside a tutor to collect data and analyze it.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings, occasional lectures
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Excellent reading and writing in English
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Ushakov; K.; Koffler-Brill; T.; Rom; A.; Perl; K.; Ulitsky; I.; Avraham; K.B. (2017) Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems. Sci. Rep.; 7:8637.
- Yizhar-Barnea; O. and Avraham; K. B. (2017) Single cell analysis of the inner ear sensory organs. Int J Dev Biol. 61:205-213. REVIEW
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