Projects
Name
Livin, a regulator of apoptosis, in health and disease
University
Israel (FIMS) - Hebrew University, Jerusalem
Domain
Hematology
Departement
Department of Hematology, Hadassah – Hebrew University Medical Center Ein Kerem Campus, Jerusalem Israel
Head
Prof. Dina Ben Yehuda
Tutor
Dr. Riki Perlman
Languages
English, Hebrew
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes Yes No Yes Yes No No No Yes Yes No
Type of Research Project
- Basic science
What is the background of the project?
The Inhibitor of Apoptosis Protein Livin is able to inhibit apoptosis induced by a variety of stimuli. We found that Livin is specifically cleaved to produce a large C-terminal truncated Livin protein (tLivin). We further demonstrated the paradoxical activity of tLivin that not only loses the anti-apoptotic activity of Livin but also induces cell death. Our previous results include In Vitro Pre-Clinical and clinical proof of concept toward the use of tLivin for cancer therapy. Our findings suggest that tLivin may serve as a novel therapeutic avenue against cancer that will overcome apoptosis resistance in malignancy. Livin plays a role in health, not only in disease. We found that the delicate balance between the anti- and pro-apoptotic activities of Livin plays a vital role in normal thrombopoiesis. Our previous work suggests that Livin could potentially be the protein necessary to enable hematopoietic cells to produce platelets in vitro as an alternative source of single donor platelets.
What is the aim of the project?
1) The implementation of targeted tLivin-based strategies for induced tumor cell death. 2) The implementation of Livin-based strategies for efficient in vitro thrombopoiesis.
What techniques and methods are used?
Tissue culture, cell biology, flow cytometry, biochemistry, protein analysis, animal model
What is the role of the student?
What are the tasks expected to be accomplished by the student?
The student is expected to perform in vitro experiments. This requires extensive tissue culture work of cell lines as well as primary cells. The cells will be subjected to experimental procedures and the student will carry the assays of the cell cultures such as various assays for apoptosis, enzymatic activity, protein levels and interactions, flow cytometry, cell staining. The student's work will be done under supervision or according to the Tutor's decision.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary reading, seminars, group meetings
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a presentation
- The student will prepare a scientific report
- The student will prepare an abstract
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Understanding cell biology. Bachelor in any of the life science. Previous experience with tissue culture and biochemistry.
Are there any legal limitations in the student’s involvement
No
All students must have lab experience.
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
- 1) Abd-Elrahman I; Hershko K; Neuman T; Nachmias B; Perlman R; Ben-Yehuda D. The inhibitor of apoptosis protein Livin (ML-IAP) plays a dual role in tumorigenicity. Cancer Res. 2009 Jul 1;69(13):5475-80.
- 2) Abd-Elrahman I; Deutsch V; Pick M; Kay S; Neuman T; Perlman R; Ben-Yehuda D. Differential regulation of the apoptotic machinery during megakaryocyte differentiation and platelet production by inhibitor of apoptosis protein Livin. Cell Death Dis. 2013 Nov 28;4:e937.
- 2) Abd-Elrahman I; Deutsch V; Pick M; Kay S; Neuman T; Perlman R; Ben-Yehuda D. Differential regulation of the apoptotic machinery during megakaryocyte differentiation and platelet production by inhibitor of apoptosis protein Livin. Cell Death Dis. 2013 Nov 28;4:e937.