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Fundamentals of stem cells Regenerative medicine research (Ain shams University)
Egypt (IFMSA-Egypt) - Ain shams University, Ain Shams
Histology Cell Biology Department – Ain shams University, Cairo, Egypt
Prof. DR/ Amany El Shawarby
Coordinator DR/Walaa Baher Mostafa DR/Gehad Ahmed DR/ Asmaa Abd Elmoniem DR/ Sarah Abd El Gawad DR/Ghada Galal
Required: English Accepted: English
Type of Research Project
- Basic science
What is the background of the project?
The utilization of stem cells in the clinical arena is one of the most exciting and innovating areas of medicine today. In this project the students will have the opportunity to learn about fundamentals of research in stem cells and regenerative medicine and will achieve new, up to date information that is applicable to modern research and therapeutic approaches in regenerative medicine. The project provides the students with a detailed introduction to the biology of stem cells, including stem cell types and sources, self renewal, differentiation and lineage commitment and the main aspects of regenerative medicine that seeks to plan new therapies for patients with severe injuries or chronic diseases.
What is the aim of the project?
1-Enable students to develop a critical appreciation of recent scientific developments in the area of stem cells and regenerative medicine and their clinical applications.
What techniques and methods are used?
Practical sessions: - The students will perform the following procedures by their own after a descriptive lecture for each procedure, observing mentors and following a check list 1- Obtaining organs (for decellularization), long bones and adipose tissue (for stem cell isolation) from animals. 2- Isolation of mesenchymal stem cells from bone marrow of rats long bone 3- Isolation of mesenchymal stem cells from rat adipose tissue. 4- Isolation of embryonic stem cells from cord blood. 5- Counting and seeding of stem cells. 6- Sub-culture of stem cells. 7- Decellularization of whole organs. 8- Staining of paraffin sections slides using different histological stains as HE, Massons trichrome and orcien.
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1- Preparation of culture media. 2- Counting of cells using a hemocytometer. 3- Isolation &Culture mesenchymal stem cells from rat bones 4- Isolation & Culture of mesenchymal stem cells from adipose tissue of rats 5- Isolation& Culture of embryonic stem cells from cord blood 6- Examination of stem cells using inverted microscope. 7- Decellularization of an organ 8- Preparation of the histological stains 9- Staining a Paraffin Section Slide 10- Preparation a presentation related to topic in Regenerative Medicine. 11- Preparation a Mini-Research proposal related to a topic in Regenerative Medicine 12- Practice Critical appraisal 13- Practice Journal Club
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes - Interactive didactic lectures: 1- Tissue formation, function and repair. 2- Basics of stem cell biology. 3- Basics of stem cells isolation and culture. 4- Basics of tissue engineering. 5- Bioscaffolds. 6- 3D bioprinting. 7- Advances in stem cells and regenerative medicine. 8-Clinical applications in Histology
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No Specific Skills are required
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields - Dental medicine students (IADS members)
- The therapeutic potential of stem cells.Philosophical Transactions of the Royal Society B: Biological Sciences. 2010;365(1537):155-163. doi:10.1098/rstb.2009.0149/
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