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Diagnostics of intestinal parasitosis in inhabitants of Kujawsko-Pomorskie province in Poland.
Poland (IFMSA-Poland) - Nicolaus Copernicus University Ludwik Rydygier Collegium Medicum, Bydgoszcz
The Chair of Medical Biology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus
Alina Woźniak, professor
Paweł Sutkowy, PhD, assistant professor, Marta Pawłowska, MSc, academic teacher
Type of Research Project
- Basic science
What is the background of the project?
The goal of the proposed study is to assess the prevalence of intestinal parasitic diseases in adults and children - inhabitants of Kujawsko-Pomorskie province in Poland. These invasions are included to “dirty hand diseases” (per os), therefore mostly occur in nursery schools. Symptoms of intestinal parasitosis may include: allergic rhinitis of unknown origin, chronic fatigue or food intolerance. Moreover, many of such invasions may be asymptomatic.
What is the aim of the project?
Epidemiological assessment of intestinal parasitosis in inhabitants of Kujawsko-Pomorskie province in Poland.
What techniques and methods are used?
The studied material (fresh stool) will be collected from children of nursery schools, their parents and other volunteers. The studied material will be also specimen of Giardia lamblia parasites from lab culture. A non- preserved stool samples will be stored in temperature below -20°C. Defrosted fecal specimen will be subjected to enzyme-linked immunosorbent assays (ELISA). Fresh fecal samples will be used to prepare direct wet mounts (saline and iodine) using supplementary techniques (flotation, sedimentation) as well as temporary mounts (hematoxylin and trichrome staining). During analyzing of fresh fecal samples the Apacor ParaSysTM system will be also used.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Student will assay the obtained fecal samples using ELISA method based on the manual in English with help of scientific supervisor. Individually, the student will have opportunity to prepare stool samples (mounts) for examination with use of optical microscope. The student will also be involved in preparation of a research article, which will present the obtained results. The contribution of student during preparation of an article will be the analysis of results and bibliography collection.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No, there will not.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: : molecular biology, immunology, laboratory diagnostics Previous experience with: working in laboratory, writing scientific articles
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Kubiak K.; et al. Giardiasis in the Warmia and Mazury province (north-eastern Poland) – an epidemiological analysis. Polish Annals of Medicine 24 (2017): 5-8.
- Lass A.; et al. Detection of Giardia intestinalis in water samples collected from natural water reservoirs and wells in northern and north-eastern Poland using LAMP; real-time PCR and nested PCR. Journal of Water and Health (2017): wh2017039.
- McHardy I.H.; et al. Detection of intestinal protozoa in the clinical laboratory. Journal of Clinical Microbiology 52 (2014): 712-720.
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