Projects
Name
Heme acquisition and trafficking in pathogenic fungi
University
Israel (FIMS) - Technion, Haifa
Domain
Genetics
Departement
Rappaport Faculty of Medicine, Technion – IIT 1, Efron St, Haifa 31096 Israel
Head
Daniel Kornitzer
Tutor
Ziva Weissman, Mariel Pinsky
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Candida albicans is a commensal fungus of human mucosal surfaces that can cause life-threatening systemic infections in immunocompromised individuals. To overcome iron limitation in the host environment, Candida albicans are able to extract iron from heme and hemoglobin, the largest iron pool in the human body, using a system that involves an extracellular cascade of soluble and cell surface-anchored hemophores conserved across many fungal pathogens, followed by endocytosis into the cell. Our goal is to achieve a comprehensive description of the Candida albicans heme internalization and trafficking pathways.
What is the aim of the project?
Candida albicans is a commensal fungus of human mucosal surfaces that can cause life-threatening systemic infections in immunocompromised individuals. To overcome iron limitation in the host environment, Candida albicans are able to extract iron from heme and hemoglobin, the largest iron pool in the human body, using a system that involves an extracellular cascade of soluble and cell surface-anchored hemophores conserved across many fungal pathogens, followed by endocytosis into the cell. Our goal is to achieve a comprehensive description of the Candida albicans heme internalization and trafficking pathways.
What techniques and methods are used?
What techniques and methods are used? Mention the steps/stages in this project. (provide examples and avoid abbreviations. Which tools will be used in general in this project) Genetic modification by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat), Cell Biology methods will include epifluorescence microscopy to follow uptake of Zn-mesoporphyrin, a fluorescent heme analog, into the cells of wild-type and mutants strains.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will learn and then carry out the molecular biology techniques required for the project at hand. Since this will be a sub-project that is part of a larger project going on at that time in the lab, the precise details are not available at this stage. It will probably involve performing a molecular cloning, transformation of DNA (Deoxyribonucleic Acid) into microorganisms, generation of mutants, analysis of phenotypes. The student's work will be observed by the tutor.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Reading material will be provided to the student
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No prior skills required. Subjects passed: Molecular biology, biochemistry, microbiology, genetics.
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Dental medicine students (IADS members)
Articles
- For the use of students considering participating in the project; further information can be found from the following articles (please add at least one specific article) Kuznets; G et al. (2014) A relay network of extracellular heme-binding proteins drives C. albicans iron acquisition from hemoglobin; PLOS Pathogens 10(10): e1004407.