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Analysis of Antimicrobial peptide P-MAP1R2B against bacterial biofilm
Portugal (PorMSIC) - University of Lisbon, Lisbon
Departamento de Biomembranas e Nanomedicina, Edificio Egas Moniz, Faculdade de Medicina da Universidade deLisboa, Lisboa, Portugal
Type of Research Project
- Basic science
What is the background of the project?
Antibiotic resistance will lead pharmaceutical companies to a new paradigm, where conventional molecules will need to be replaced. As matter of fact, the World Health Organization has already pointed out the urgency in find new molecules against different pathogens, named the ESKAPE group (Enterococcus faecium, Staphylococcus aureus, Klebsiellapneumoniae, Acinetobacterbaumanii, Pseudomonas aeruginosa and Enterobacter species). Antimicrobial peptides (AMPs) are considered an alternative for infectious therapies. Being small, cationic and hydrophobic, their major advantage is the difficulty for pathogens to acquire resistance. In the present work an evaluation of PAMP1R2B activity against bacterial biofilm will be studied. Inhibition and eradication assays combined with kinetics of biofilm lifetime in the absence and in the presence of the synthetic AMP will be evaluated.
What is the aim of the project?
The project aims to determine the captability of P-AMP1R2B to act against bacterial biofilm.
What techniques and methods are used?
The project will make use of microbiology techniques, such as absorbance for inhibition, eradication and biofilm lifetime determination. The other steps of the project are: 1. Optimization of biofilm growth conditions by determining the time dependence and the bulk mass of the bacterial biofilm growth 2. Determination of optical density of bacterial cells after treatment with the antimicrobial peptide 3. Correlation between the obtained data and the kinetic of biofilm formation He project is divided in two parts, since we want to evaluate the effects in the absence and in the presence of the synthetic AMP (antimicrobial peptides). This way we will be able to evaluate PAMP1R2B activity against bacterial biofilm.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to be able to work with microorganisms and bacterial cells: culture, growth cycle, treatment. He will also be involved in the optimization of biofilm growth conditions by determining the time dependence and the bulk mass of the bacterial biofilm growth, determination of optical density of bacterial cells after treatment with the antimicrobial peptide and correlate the obtained data with the kinetic of biofilm formation. Once the optimization for biofilm growth has been determined, inhibition and eradication properties of the peptide will be studied. Finally, the student will be involved in the development of a presentation about the results and conclusions of the project.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No theoretical teaching will be provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic laboratory equipment manipulation ( pipeting, transferring, data treatment). Previous experience with laboratory techniques.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- SuzanaM.Ribeiroa; MárioR.Felício; EstherVilasBoas; SóniaGonçalves; FabrícioF.Costa; RamarPerumalSamy; NunoC.Santos; OctávioL.Franco; New frontiers for anti-biofilm drug development; https://doi.org/10.1016/j.pharmthera.2016.02.006
- SóniaGonçalves; Patrícia M. Silva;Mário R. Felício; Luciano N. de Medeiros;EleonoraKurtenbach and Nuno C. Santos;Psd1 Effects on Candida albicans Planktonic Cells and Biofilms; doi: 10.3389/fcimb.2017.00249
- LudovicoMigliolo; MárioR.Felício; Marlon H.Cardoso; OsmarN.Silva; Mary-Ann E.Xavier; Diego O.Nolasco; AdelianaSilvade Oliveira; IgnasiRoca-Subira; Jordi Vila Estape; Leandro D.Teixeira; Sonia M.Freitas; AnselmoJ.Otero-Gonzalez; SóniaGonçalves; NunoC.Santos; Octavio L.Franco; Structural and functional evaluation of the palindromic alanine-rich antimicrobial peptide Pa-MAP2; https://doi.org/10.1016/j.bbamem.2016.04.003
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