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Characterization of intercellular signaling pathways involved in chick lung development
Portugal (PorMSIC) - University of Minho, Braga
Life and Health Sciences Research Institute (ICVS) Universidade do Minho Campus de Gualtar 4710-057 Braga Portugal
Jorge Correia Pinto
English, Portuguese, Spanish
Type of Research Project
- Basic science
What is the background of the project?
Lung development is directed by intrinsic epithelial-mesenchymal interactions that regulate cell proliferation, fate, migration and differentiation, leading to branching morphogenesis and ultimately to proper lung formation. The intricate mechanisms that determine a correct lung development depend on the involvement of several paths. Aiming to untangle an association between these signaling pathways in chick lung morphogenesis, inhibitors or activators of these signaling pathways will be added to chick lung explants culture to evaluate their effect in lung morphology. With this project, we expect to confirm the relationship between regulators of lung development (Fibroblast Growth Factor - FGF, Notch, Sonic hedgehog - SHH and retinoic acid members), disentangling its potential role in the overall process of fetal lung morphogenesis.
What is the aim of the project?
The aim of the project is to characterize the expression pattern of the members of known molecular players involved branching, in the chick model and to dissect the links between different signaling pathways
What techniques and methods are used?
The techniques used in the project are microsurgery;in situ hybridization; in vitro lung explants culture and morphometric analysis. First, the project will collect samples of lung tissue. Then, it will add inhibitors or activators of the signaling pathways associated with lung development. As with every project, it's important to analise the data collected and conclued if there is a association between these signaling pathways and different lug development regulators.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to learn microsurgical techniques in order to collect samples of lung tissue. He is also expected to learn the basic molecular biology methods needed in the project such as in situ hybridization, microbiology techniques and lung explant culture. Depending on the level of understanding of each technique and the stage of the project, the student will be able to perform them under supervision. The student will also participate in the interpretation and analisis of the data collected necessary to see if there is an association between these signaling pathways and different lug development regulators.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No theoretical teaching will be provided for the student. The student is encouraged to read the article mentioned.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No special skills required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Fernandes-Silva H; Vaz-Cunha P; Barbosa VB; Silva-Gonçalves C; Correia-Pinto J; Moura RS Retinoic acid regulates avian lung branching through a molecular network. 2017. Cell Mol Life Sci; 74(24):4599-4619. doi:10.1007/s00018-017-2600-3.
- Rute S. Moura ; José P. Coutinho-Borges; Ana P. Pacheco; Paulo O. daMota; Jorge Correia-Pinto FGF Signaling Pathway in the Developing Chick Lung: Expression and Inhibition Studies 2011. PLoS ONE; 6(3):e17660. doi:10.1371/journal.pone.0017660
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