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(Cluj-Napoca) Humoral factors in urate-dependent cytokine production
Romania (FASMR) - University of Medicine and Pharmacy "Iuliu Hatieganu", Cluj-Napoca
Dr. Radu Anghel Popp
Tania Crisan, Medeea Badii
Type of Research Project
- Basic science
What is the background of the project?
Gout is the most common arthritis in men about 40 years of age, and the prevalence of gout is ever increasing. The deposition of uric acid monosodium crystals in joints induces inflammation, which is classical for gout. However, a growing body of evidence suggests that increased concentrations of uric acid also contribute to other inflammatory/metabolic diseases (e.g. metabolic syndrome, atherosclerosis). The mechanisms through which uric acid enhances the inflammatory setpoint of the immune system are unknown.
What is the aim of the project?
The objective of this project is to assess whether sera of patients with varying uric acid concentrations induce reprogramming in cells of innate immune system, leading to variation in inflammatory state.
What techniques and methods are used?
• peripheral blood mononuclear cellswill be isolated from blood of healthy volunteers or patients • serum separation will be performed from blood of donors • primary cell culture and stimulation experiments will be performed using medium supplemented with autologous or heterologous serum • microscopy and colorimetric assays will be performed to determine subsequent cell phenotype, urate level or cytokines in cell culture supernatants.
What is the role of the student?
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to shadow the supervisor, take notes of procedures, and learn protocols. Thereafter, the student will perform his/her own experiments under supervision or individually. They will need to know how to work in a sterile cabinet, how to perform a cell culture, how to calculate amounts and concentrations of conditions needed to apply over cells. Following up on this, they will need to know how to handle a microscope and assist the investigator for quantitative readout such as ELISA(enzyme-linked immunosorbent assay) or other colorimetric assays. Use of labjournal and fore-planning will be required.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No. We will provide guidance and protocols once the student is in the lab. The student is encouraged to read about gout and hyperuricemiato build his/her general background knowledge.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
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