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Establishment of Alzheimer Disease model induced by ApoE4 in zebrafish.
China (IFMSA-China) - Southern Medical University, Guangzhou
Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University. Guangzhou, China
WANG fang (supervisor of the research group)
Type of Research Project
- Basic science
What is the background of the project?
ApoE4 is the strongest genetic risk factor for late-onset Alzheimer disease. ApoE4 increases brain amyloid-β pathology relative to other ApoE isoforms. Carriers of the ApoE ε4 allele have more rapidly progressive memory loss and reduced learning efficiency in their 50s and 60s than do those who do not carry the ApoE ε4 allele (non carriers). The construction of animal models contributes to the understanding of Alzheimer's Disease. Once the AD zebrafish model is available, it could be, by far, the most cost-effective vertebrate that can be used for drug screening, due to its rapid generation time and high fecundity.
What is the aim of the project?
To establish Alzheimer Disease model induced by ApoE4 in zebrafish. To learn the mechanism of Alzheimer Disease.
What techniques and methods are used?
The techniques, such as CRISPR-Cas9, Immunohistochemistry, Western blot and qPCR etc. will be used in the project. CRISPR is a family of DNA (deoxyribonucleic acid) sequences in bacteria. The sequences contain snippets of DNA from viruses that have attacked the bacterium. These snippets are used by the bacterium to detect and destroy DNA from similar viruses during subsequent attacks. These sequences play a key role in a bacterial defense system, and form the basis of a technology known as CRISPR/Cas9 that effectively and specifically changes genes within organisms. Images were analyzed using FIJI (open source) or Imaris (ver. 7, Bitplane) software, respective TIFF files generated and figures assembled in Adobe Photoshop (ver. CS5).
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The tasks that the student should be accomplished by he/her self include: understanding the basic mechanism and performing the experiment of real time PCR(Polymerase Chain Reaction) to analyze the related genes involve in the zebrafish model. Also the student will be involved in protein extraction and purification followed by identification of them with Western blot(WB) technique by he/her self.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, the preliminary readings and lectures will be provided after the student come in the lab.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The techniques, such as CRISPR-Cas9, Immunohistochemistry, Western blot and Qpcr(Polymerase Chain Reaction) etc. will be used in the project.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Exp Gerontol. 2017 Dec 21. pii: S0531-5565(17)30541-7. doi: 10.1016/j.exger.2017.12.019. [Epub ahead of print] Role of the peripheral innate immune system in the development of Alzheimer's disease. Le Page A(1); Dupuis G(2); Frost EH(3); Larbi A(4); Pawelec G(5); Witkowski JM(6); Fulop T(7).
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