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CCL4 and CCL7 mediate the intestinal barrier dysfunction in LPS-induced sepsis in mutant mice
China (IFMSA-China) - Southern Medical University, Guangzhou
Department of Pathophysiology, State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Proteomics, Southern Medical University, GuangZhou,China
Yong Jiang, PhD
Type of Research Project
- Basic science
What is the background of the project?
Chemokine(C-C motif) ligand 4( CCL4), is a protein which in humans is encoded by the CCL4 gene. Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine. Sepsis is a systemic inflammatory state caused by infection, which is characterized by the release of pro-inflammatory mediators. Studies have demonstrated that the integrity of the gut epithelium and competence of adaptive immune responses are notoriously compromised during sepsis. Tight junction dysregulation and epithelial damage contribute to barrier loss in sepsis. However, the mechanisms that regulate these processes and their relative contributions to sepsis are not completely understood. We assessed that the mechanism of barrier dysfunction mediates the sepsis in LPS( lipopolysaccharide)-induced model.
What is the aim of the project?
Our aim was to investigate mechanism that ccl4 and ccl7 activite intestinal barrier dysfunction and mediate the sepsis in LPS-induced mice.
What techniques and methods are used?
Protein expression analysis biochemical analysis. Fecal albumin content Epithelial cell and lamina propria cell isolation. RNA(ribonucleicacid) extraction and realtime PCR analysis. RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA. Several methods are used in molecular biology to isolate RNA from samples, the most common of these is Guanidinium thiocyanate-phenol-chloroform extraction. The filter paper based lysis and elution method features high throughput capacity. RNA extraction in liquid nitrogen, commonly using a mortar and pestle (or specialized steel devices known as tissue pulverizers) is also useful in preventing ribonuclease activity. SPSS will be used to analyze the data. All values are presented as the mean ± S.E.M
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
What are the tasks expected to be accomplished by the student?
Basic medical sciences experiments: PCR(Polymerase Chain Reaction), Western Blot, et al. RNA extraction and realtime PCR analysis. The student will be assigned to do real-time PCR on his or her own . Sometimes, the student may need to prepare facility for other members of this laboratory and do some cleaning when he or she is on duty.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- 1. Aliment Pharmacol Ther. 2014 Nov;40(10):1187-201. doi: 10.1111/apt.12950. Epub 2014 Sep 28. Review article: intestinal barrier dysfunction and central nervous system disorders--a controversial association. Julio-Pieper M(1); Bravo JA; Aliaga E; Gotteland M.
- 2. Am J Transl Res. 2017 Nov 15;9(11):5040-5047. eCollection 2017. Dexmedetomidine alleviates LPS-induced septic cardiomyopathy via the cholinergic anti-inflammatory pathway in mice. Kong W(1); Kang K(1); Gao Y(2); Liu H(3); Meng X(1); Yang S(1); Yu K(3)(4); Zhao M(1).
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