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Search for Leishmania spp. In sandflies, dogs and humans with suspected leahsmaniasis.
Federal University of Grande Dourados – UFGD
Faculty of Heath Sciences/UFGD. Rodovia Dourados – Itahum Km 12. Cidade Universitária. Dourados – MS – Brasil. CEP: 79.804-970.
Silvia Aparecida Oesterreich
Herintha Coeto Neitzke Abreu
English and Portuguese
Type of Research Project
- Basic science
What is the background of the project?
Leishmaniasis is as zoonotic disease with a high public heath impact. The involvement of sandflies in the transmission of leishmaniasis show the importance of investigations of the fauna, behavior and infection rate of Leishmania spp., because the information deficit in the State of Mato Grosso do Sul makes it difficult to develop control measures. The dog is the main urban reservoir of leishmaniasis. For diagnosis, different methods have been suggested, including molecular biology techniques such as a Polymerase Chain Reaction (PCR). The identification of the infecting species is important, since differences in the etiological agents are related to the different degrees of virulence and pathogenicity, which may interfere in the specific therapeutics. Thus, the research and identification of Leishmania in dogs and humans are important in the epidemiology of leishmaniasis.
What is the aim of the project?
The objective is to investigate the fauna of sandflies, the behavior of these insects and the rate of natural infection by Leishmania spp. Finally, the project aims to research Leishmania spp. In different clinical samples of dogs for knowledge of the dissemination of the parasite.
What techniques and methods are used?
Collection of sandflies with Centers for Disease Control (CDC) and Falcao traps; Identification of the species under an optical microscope; Collection of biological samples from humans and dogs; Polymerase Chain Reaction (PCR) performed to investigate Leishmania in sandflies, humans and dogs; Polymerase Chain Reaction (PCR) – Restriction Fragment Length Polymorphism (FRLP) for the identification of Leishmania species.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
To collect sand flies in rural and urban areas; To identify the sandflies species through dissection and observation under as optical microscope; Processing of the biological samples of dog and human; Perform Polymerase Chain Reaction (PCR) in different clinical samples (DNA extraction, amplification with different primer and electrophoresis for the development of bands ) and Restriction Fragment Length Polymorphism (RFLP).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes. Initially, the student will be guided from the laboratory routine. Then, they will know the objectives of the project and will accompany undergraduate and graduate students in the experiments. A seminar will be held detail the Polymerase Chain Reaction (PCR) design and technique.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Manipulation of biological material and use of micropipettes.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields
- -NEITZKE- ABREU HC; REINHOLD-CASTRO KR; VENAZZI MVS; SCODRO RBL; DIAS AC; SILVEIRA TGV et al. Detection of Leishmania (Viannia) in Nyssomyia neivai and Nyssomyia whitmani by Multiplex Polymerase Chain Reaction; in southern Brasil. Revista do Instituto de Medicina Tropical de São Paulo; 56(5):391-395; 2014.
- -Neitzke- Abreu HC; Venazzi MS; Bernal MVZ; Reinhold Castro KR; Vagetti F; et al. Detection of DNA from Leishmania (Vianniaa): accuracy of polymerase chain reaction for the diagnosis of cutaneous leishmaniasis. PLos ONE; 8(7):e62473; 2013.
- -NEITZKE; HC; et al. Pequiesa de infecção natural de flenotomíneos por Leishmania no estado do Paraná; Brasil. Revista da Sociedade Brasileira de Medicina Tropical; v. 41; p. 17-22; 2008
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