Population genetic diversity of Schistosoma japonicum among host using microsatellites by multi- Polymerase Chain Reaction (PCR)
China (IFMSA-China) - Wuhan University School of Medicine, Wuhan
Department of Parasitology, School of Basic Medical Science, Wuhan University
Hui-Fen Dong
Qin-Ping Zhao
English, Chinese
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No No Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Schistosomiasis is one of the most neglected tropical diseases with more than 200 million people infected in the world. Schistosoma japonicum was thought to be the most virulent species among six Schistosoma, because of its high egg production and over 40 kinds of livestock and wild animals can be as definitive hosts. The epidemiological and transmission pattern of S. japonicum may be changed after long term control, especially large amounts of buffaloes prohibited in endemic areas. The population genetic diversity of S. japonicum by host may be changed, which then lead the change of transmission pattern of S. japonicum in the field.
What is the aim of the project?
Find out the genetic diversity of population of S. japonicum among different hosts, to illuminate the transmit pattern of disease after long-term infectious source control.
What techniques and methods are used?
1. Extract the genomic DNA full form from single cercariae and miracidium of Schistosoma japonicum stored on Whatman FTA card. 2. Multi-Polymerase Chain Reaction (PCR ) amplification for microsatellites for each single larva. 3. Data collection from sequencing by using Geneious. 4. Genetic diversity analysis.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be expected to know how to collect single larva onto whatman FTA full form cards for long term DNA storage in room temperature. Use the DNA extraction method for single larva, which is improved and modified in my lab for this project. Also The student should understand the whole process of repeatable multi- Polymerase Chain Reaction (PCR ) amplification for microsatellites from single larva or genome-wide SNP full form amplification. He/she should try to use some bioinformatics software, such as Geneious, Microchecker, phyML, DNAsp full form, Mega, and Arlequin to analyze the genetic diversity and phylogenetic relationship among populations.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, we will have a lab meeting per week, mainly focus on the process of lab work, orally or in presentation.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
reference reading, Polymerase Chain Reaction (PCR ), blast, DNA extraction
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Students in biomedical fields