Effects of interaction between moesin and CD44 on pericyte loss and immature neovessel formation in diabetes.
China (IFMSA-China) - Southern Medical University, Guangzhou
Department of Pathophysiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, P.R.China
Yong Jiang, PhD
Qiaobing Huang, PhD
English, Chinese
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes Yes Yes Yes Yes
Type of Research Project
- Basic science
What is the background of the project?
This project proposed that advanced glycation end products (AGEs) is involved in the development of abnormal diabetic angiogenesis by inducing the interaction of threonine phosphorylation of moesin and CD44. The binding of phosphorylation of moesin and CD44 will mediates the migration of pericyte, the disruption of cross-talk between endothelium and pericyte, leading to the pathological angiogenesis.
What is the aim of the project?
This project aims at first to elucidate the involvement (expression and phosphorylaiton) of moesin and CD44 in the progress of pericyte migration, the inteaction between endothelium and pericyte, and then to explore the moesin-related mechanism of AGE-induced diabetic proliferative microangiopathy. The signal pathway of this moesin-related mechanism will be investigated too.
What techniques and methods are used?
Primary culture of retinal microvascular pericyte; Quantification of pericyte proliferation; Cell migration assay; Observation and quantification of angiogenesis assay system in rat aortic rings; Transfection of target siRNA (Small interfering RNA)to pericyte;… etc. The proliferation, migration and recruitment of pericytes by endothelium are critical processes in mature neovessel formation. These above-mentioned techniques are important parameters for the observation and evaluation of angiogenesis in vitro, as well as in vivo. By using these techniques, we can judge the effect of interaction between moesin and CD44 in different period of angiogenesis.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be assigned to culture pericytes with or without intervention, then to use matrigel as medium to observe and analysis the colocalization of pericyte and endothelial. The student will also be assigned to observe and quantify the angiogenesis of rat aortic rings. The first thing the student needs to do is to learn cell culture. And then to learn how to observe migration of pericyte. For the intervention of the cells, the student needs to know (or learn) siRNA and gene mutant technique. For the observation of angiogenesis of rat aortic rings, the student needs to learn how to handle a rat, to isolate the aorta and, to obtain aortic rings for tissue culture.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be some seminars about the projects.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
- The student will have the opportunity to present the results together with the supervisor at a conference
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student had better has obtained the technique of cell culture.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Xu Y; Wang L; He J; Bi Y; Li M; Wang T; Wang L; Jiang Y; Dai M; Lu J; Xu M; Li Y; Hu N; Li J; Mi S; Chen CS; Li G; Mu Y; Zhao J; Kong L; Chen J; Lai S; Wang W; Zhao W; Ning G; 2010 China Noncommunicable Disease Surveillance Group. Prevalence and control of diabetes in Chinese adults. JAMA. 2013 Sep 4;310(9):948-59.
- Ip MS; Domalpally A; Sun JK; Ehrlich JS. Long-term effects of therapy with ranibizumab on diabetic retinopathy severity and baseline risk factors for worsening retinopathy. Ophthalmology. 2015 Feb;122(2):367-74.
- Das A; McGuire PG; Rangasamy S. Diabetic Macular Edema: Pathophysiology and Novel Therapeutic Targets. Ophthalmology. 2015 Jul;122(7):1375-94.
- Kohno R; Hata Y; Mochizuki Y; Arita R; Kawahara S; Kita T; Miyazaki M; Hisatomi T; Ikeda Y; Aiello LP; Ishibashi T. Histopathology of neovascular tissue from eyes with proliferative diabetic retinopathy after intravitreal bevacizumab injection. Am J Ophthalmol. 2010 Aug;150(2):223-229.
- Cheng R; Ma JX. Angiogenesis in diabetes and obesity. Rev Endocr Metab Disord. 2015 Mar;16(1):67-75.
- Wang Q; Fan A; Yuan Y; Chen L; Guo X; Huang X; Huang QB. Role of Moesin in Advanced Glycation End Products-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells. Sci Rep. 2016 Mar 9; 6:22749.
- Wang J; Liu H; Chen B; Li Q; Huang X; Wang L; Guo X; Huang QB. RhoA/ROCK-Dependent Moesin Phosphorylation Regulates AGE-induced Endothelial Cellular Response. Cardiovasc Diabetol. 2012 Jan 17;11(1):7.