Projects
Name
Prospective survey of acute osteoarticular infections in a Moroccan pediatric orthopedic surgery unit.
University
Morocco (IFMSA Morocco) - Faculty of medicine and pharmacy of Fes, Fes
Domain
Pediatrics
Departement
Microbiology and molecular biology laboratory, Faculty of medicine and pharmacy of Fez. Pediatric orthopedic surgery department, Hassan II University Hospital of Fez.
Head
Pr. Moulay Abderrahmane and Pr. Bouchra Oumokhtar
Tutor
Dr Kaoutar Moutawakkil ; bouchra.oumokhtar@usmba.ac.ma
Languages
English, French
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No Yes Yes Yes No No No No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Osteo-articular infections (OAI) remain a significant cause of morbidity worldwide in young children. They can have a devastating impact with a high rate of serious and long-lasting sequelae, especially on remaining growth. The diagnosis and treatment must be undertaken urgently because it can bring into play the functional and even the vital prognosis of the child. The etiologic diagnosis was based on blood cultures, joint aspirations and bone punctures. Two bacteria are particularly implicated: S. aureus with Methicillin-resistant community strains and secretory strains of the dreaded Panton Valentine toxin responsible for particularly severe paints, as well as; Kingella kingae who even became the first germ responsible for septic arthritis. We conducted a prospective study to analyze the recent epidemiology and the clinical evolution of pediatric OAI in order to validate the adequacy of our probabilistic first-line antibiotic treatment.
What is the aim of the project?
The aim of the project is to study the epidemiology and the microbiology of bone and joint infections in young children, as well as clinical presentations, diagnosis, and treatment of these infections at the university hospital center Hassan II, Fez.
What techniques and methods are used?
The samples collected are blood cultures, joint aspirations and bone punctures. Microbiological Molecular analysis: the diagnosis of Staphylococcus aureus, Kingella kingae, Streptococcus pneumoniae and Streptococcus pyogenes is to be performed. The identification of bacteria is made through biochemical testing such as Catalase test and Pyrrolidonyl Aminopeptidase (PYR) (for the detection of pyrrolidonyl arylamidase), and the susceptibility testing will be by antibiogram according to international criteria. Genes encoding S.aureus, Panton-Valentine toxin, methicillin resistance, and kingella kingae was investigated by Polymerase chain reaction (PCR).
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will observe the different sample collection methods before partaking in the process while under supervision (blood and joint tissue sampling). Same goes for microbiological molecular analysis techniques (The most important one being polymerase Chain Reaction (PCR); a technique that can enzymatically amplify minute quantities of DNA or RNA to large number of copies (≥107 copies) in a short period hence providing ample target that can be readily detected). the student will practice some of those said techniques (mainly bacteria identification and antibiogram analysis) and then input the different results in the lab’s data collector.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, mainly lectures delivered by the head of department or one of the assistant professors to introduce the students to the basics of microbiology and lab analysis.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No skills in particular are required.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
- Prospective survey of acute osteoarticular infections in a French paediatric orthopedic surgery unit.”; Ferroni A; Al Khoury H; Dana C; Quesne G; Berche P; Glorion C; Péjin Z. (https://www.ncbi.nlm.nih.gov/pubmed/23957786).
- “Osteoarticular Infections in Children.”;Arnold JC; Bradley JS. (https://www.ncbi.nlm.nih.gov/pubmed/26311358
- “Osteoarticular infections due to Kingella kingae in children”; M. Lacour; M. Duarte; A. Beutler; R. Auckenthaler; S. Suter. (https://link.springer.com/article/10.1007/BF02072618).