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The effect of metformin and aspirin in pancreatic cancer cells in vitro
Portugal (PorMSIC) - University of Porto, Porto
Unit of Biochemistry, Department of Biomedicine; Alameda Prof. Hernâni Monteiro 4200–319 Porto Portugal
José Castro Lopes
Type of Research Project
- Basic science
What is the background of the project?
Pancreatic cancer, as the fourth leading cause of cancer-related deaths, carries a poor prognosis with a median survival of 6 months and a dismal 5-year survival rate of 3% to 5%. These statistics highlight an urgent need for novel chemopreventive and therapeutic strategies for this malignancy. Metformin and aspirin have been explored as two emerging cancer chemoprevention agents for different types of cancers, including pancreatic cancer. With this line of thought, this project will study the effect of this drugs in pancreatic cancer cells in vitro.
What is the aim of the project?
The aim of this project is to investigate the effect of metformin and of aspirin, alone and in combination, on the growth, viability and migration capacity of pancreas cancer cell lines.
What techniques and methods are used?
The project will start with the collection of samples and preparation in vitro cell. To analyse the effects of metformin and aspirin in the pancreatic cells, it will be used SRB assay – sulforhodamine B assay, to quantificate the culture growth, incorporation of 3H-thymidine to assess cell proliferation, extracelular LDH - Lactate dehydrogenase activity assay to acess the viability and injury assay to analyse migration. With this four techniques, we will be able to characterize the efects of metformin and aspirin, alone and in combination, in the pancreatic cells.
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will learn some simple methods (like the ones mentioned in the next paragraph) for in vitro analysis of cell proliferation, viability and migration. He is expected to participate in performance of the lab techniques, such as in vitro cell culture, quantification of culture growth (SRB assay – sulforhodamine B assay), cell proliferation (incorporation of 3H-thymidine), viability (extracelular LDH - Lactate dehydrogenase - activity assay)) and migration (injury assay).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No special skill is required
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- “Drug repurposing in cancer“ Linda Sleirea; Hilde Elise Førde; Inger Anne Netlanda; Lina Leiss; Bente Sandvei Skeie; Per Øyvind Enger.
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