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Investigation of cellular processes which participate in the anti-tumor response of Calixarene in pancreatic adenocarcinoma cells (Panc-1) from analysis of microRNA expression.
Brazil (IFMSA Brazil) - Centro Universitario CESUMAR (UNICESUMAR), Maringa
Medicine department Av. Guedner, 1610 - Jardim Aclimacao, Maringá - PR, 87050-900
Nilce Marzolla Ideriha
Karin Juliane Pelizzaro Rocha Brito
Type of Research Project
- Basic science
What is the background of the project?
Pancreatic cancer is one of the most common neoplasms of the gastrointestinal tract. Practically no progress has been made to diagnose and treat patients with early stage tumors. Besides this, aggressiveness of pancreatic cancer has been linked to elevated levels of pro-survival mediators. In order to obtain drugs more effective, specific and with lower side effects, a better knowledge about the tumor biology, as well as the candidate of drug under the molecular aspect, is necessary. In a preliminary study, Pelizzaro-Rocha et al.(2013) demonstrated that calixarene (CLX6) acts as a powerful agent against the aggressiveness of pancreatic cancer. Thus, the project proposes to provide information and global biochemical correlations on the performance of the CLX6 in the metabolism of pancreatic cancer cells, specifically on the miRNAs expression, predict side effects and the applicability of this compound in the treatment of other types of tumors.
What is the aim of the project?
Evaluate the role of 186 miRNAs modulated by CLX6 in signaling pathways using a survey approach from data available in databases.
What techniques and methods are used?
Currently thousands of interactions between miRNA and genes have been experimentally identified. We will used databases as DIANA-TarBase 7.0 (Vlachos et al, 2014), that is a database that stores an experimentally verified collection of miRNA targets in different species. The DIANA-TarBase v7.0 user can easily perform an analysis of cellular processes and/or signaling pathways for miRNA (s) under investigation through DIANA-miRPath v3.0 (Vlachos et al., 2015). This database was significantly extended to support all analyzes for KEGG molecular pathways, as well as multiple cellular processes classified by Gene Ontology (GO) in seven species (Homo sapiens, Mus musculus, Rattus norvegicus, Drosophila melanogaster, Caenorhabditis elegans, Gallus gallus e Danio rerio). DIANA-miRPath v3.0 which allows users to identify and visualize important miRNAs for specific cellular processes based on in silico or experimental data.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to evaluate the role of 186 miRNAs modulated by CLX6 in signaling pathways using a survey approach from data available in databases, such as DIANA-TarBase 7.0 and DIANA-miRPath v3.0. He/she will also do readings in the course of the project to grow his/her knowledge about the subject. It is expected that the student have critical spirit to evaluate data and to do researches, commitment to the project and know how to work in small group.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, the student will be estimulated to do previous readings and readings in the course of the project. Besides that, there will be weekly moments when the student will be able to question and present his/her hypotheses to the tutor.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Subjects passed: Cell and molecular biology. Previous experience with: basic science .
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields - Dental medicine students (IADS members)
- Karin Juliane Pelizzaro-Rocha; Marcelo Bispo de Jesus; Roberta Regina Ruela-de-Sousa; Celso Vataru Nakamura; Fabiano Souza Reis; Angelo de Fátima; Carmen Veríssima Ferreira-Halder. Calixarene bypasses human pancreatic cancer aggressiveness: downregulation of receptor tyrosine kinases and induction of cell death by reticulum stress and autophagy. Biochimica et Biophysica Acta – Molecular Cell Research. 1833: 2856–2865; 2013.
- Júlia L Abrantes; Thaís F Tornatore; Karin J Pelizzaro-Rocha; Marcelo B de Jesus; Rodrigo T Cartaxo; Renato Milani; Carmen Veríssima Ferreira-Halder. Crosstalk between kinases; phosphatases and microRNAs in cancer. Biochemie. 107: 167-187. 2014.
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