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Molecular profiling of gene expression and epigenetic characterisation of lung cancer cells in Bulgarian patients for identification of possible genetically specific therapies
Bulgaria (AMSB) - Medical University of Sofia, Sofia
Molecular Medicine Center, Department of Medical Chemistry and Biochemistry, Medical University of Sofia,
Academician Prof. Vanio Mitev, MD, PhD, DSc
Assoc. Prof. Radka Kaneva, PhD; Chief Assist. Prof. Atanaska Mitkova, Darina Kachakova, PhD, Silva Giragosyan, PhD student , Veronika Petkova, PhD student
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Lung cancer (LC) is the leading cause of cancer related mortality worldwide and belongs to the group of the so-called socially significant diseases. itself) (min. 10 lines, max. 20 lines) Classically, treatment decisions have been empiric and based upon histology of the tumour. Modern genetic, epigenetic and genomic studies of lung carcinomas revealed specific molecular profiles and expression patterns of Micro Ribonucleotide Acid (miRNAs). MiRNAs are small (21-23 bp) endogenous non-coding RNA molecules. They regulate the gene expression at post-translational level, by binding to the template RNA (mRNA) or by degradation or translational repression. MiRNAs are potential markers for the study of coordinated gene expression since they can regulate several genes. By studying these epigenetic processes, we aim to suggest corresponding specific therapies.
What is the aim of the project?
The aim of the project is to identify the epigenetic and gene expression characteristics of lung cancer cells in Bulgarian patients as basis for genetically specific therapies.
What techniques and methods are used?
1. Isolation of RNA molecules from different types of lung cancer cells. 2. Quantity and quality analysis of the isolated RNA molecules. 3. Analysis of the acquired data. Techniques and methods include: RNA isolation, RNA quantitation, Reverse transcription, Quantitative Polymerase chain reaction (qPCR), Basic statistical tools and data analysis.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be expected to take active part in the research project. This will include review of relevant literature on his/her own, participation in scientific discussions and seminars, practical tasks that will include the methods listed previously (RNA isolation, RNA quantitation, using Reverse transcription, setting up Quantitative Polymerase chain reaction ) and learning about basic statistical and analytical methods of analysis. Towards the end of the exchange period the student will be expected to present a scientific report about the project and his participation.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings , seminars and hands-on lab training will be included.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student would need to have taken university level courses in genetics and biochemistry and/or molecular biology. Good command of the English language is a must, as well as basic computer skills (Windows OS, Microsoft Office – Word, Excel, Power Point). Experience with the most common molecular biology databases, e.g. PubMed, Online Mendelian Inheritance in Man (OMIM), The Exome Aggregation Consortium (ExAC) and Ensembl, is a plus. Subjects passed: university level courses in genetics and biochemistry and/or molecular biology Previous experience with: molecular genetic techniques and use of most common molecular biology databases, e.g. PubMed, Ensemble, the microRNA sequence database (miRBase) etc., is a plus
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Rothschild SI (2013) Epigenetic Therapy in Lung Cancer - Role of microRNAs. Front Oncol. 19;3:158. doi: 10.3389/fonc.2013.00158. eCollection 2013
- Del Vescovo; V.; Grasso; M.; Barbareschi; M.; & Denti; M. A. (2014). MicroRNAs as lung cancer biomarkers. World Journal of Clinical Oncology; 5(4); 604–620.
- Tamura; T.; Asamura; H.; Kawaishi; M.; Kuwano; K.; Ochiya; T.; 2015. The Clinical Relevance of the miR- 197/CKS1B/STAT3-mediated PD-L1 Network in Chemoresistant Non-small- cell Lung Cancer. Mol. Ther. 23; 717–727
- Schreiber; R.D.; Old; L.J.; Smyth; M.J.; 2011. Cancer immunoediting: integrating immunity’s roles in cancer suppression and promotion. Science 331; 1565–1570.
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