Projects
Name
Hemoglobin microparticles – characterization and study of their application as artificial oxygen carriers
University
Bulgaria (AMSB) - Medical faculty of Trakia University, Stara Zagora
Domain
Biophysics and Medical Physics
Departement
Department of Physics
Head
Miroslav Karabaliev
Tutor
Miroslav Karabaliev
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The project is related with application for save blood transfusion.It can be used in clinically conditions and in extreme situations .Recent studies have shown that the majority of these problems can be overcome by using integrated micro- or nanoparticles, preparated from hemoglobin .The objectives are exploring in vitro some physicochemical properties of the hemoglobin microparticles (Hb-MPs) and their interactions with erythrocytes in the blood. The fabrication procedure provides particles with a narrow size distribution and nearly uniform morphology. The content of hemoglobin (Hb) in the particles corresponded to 80% of the Hb content in native erythrocytes. Furthermore, we demonstrate a successful perfusion of isolated mouse glomeruli with concentrated HbP suspensions in vitro. A normal, nonvasoconstrictive behavior of the afferent arterioles is observed, suggesting no oxygen oversupply and limited NO scavenging by these particles, making them a highly promising blood substitute.
What is the aim of the project?
The aim is to investigate various properties and characteristics of Hb-MPs .The scientific tasks are to determinate the redox potencial and the cooperativnes of the process of electron transfer Hb-MPs and to compare it with those of Hb,to investigate the influence of various substances in the electrolyte solution on the values of the redox potencial of Hb and Hb-MPs,to study the effects of Hb-MPs on the eritrocytes.
What techniques and methods are used?
Preparation of Hb-MPs by the technique of coprecipitacion of Hb and MnCO3(Manganese(II) carbonate) ,subsequent addition of albumin and cross-linking by glutaraldehyde. Measurement of the redox potencial of Hb and Hb-MPs by spectroelectrochemistry .Determination of the influence of Hb-MPs on the stability of erythrocytes – by acid haemolysis and hypotonic haemolysis ,monitored by spectrophotometry and investigation of the thermal stability of the erythrocytes by impedance spectroscopy
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student has to follow the rules in the lab and the instructions of the tutor and his assistants . He/She will be monitored by the assistants and together they will work on preparation of Hb-MPs by the technique of coprecipitacion of Hb and MnCO3 .Subsequent addition of albumin and cross-linking by glutaraldehyde. Measurement of the redox potential of Hb and Hb-MPs by spectroelectrochemistry .Determination of the influence of Hb-MPs on the stability of erythrocytes. Investigation of the thermal stability of the erythrocytes by impedance spectroscopy.Other task – exploring the other effects of Hb-MPs on erythrocytes. .Making a clear correlation between the values of the redox potencial of free Hb and of Hb particles . Searching possible presence or absence of side effects of Hb-MPs on red blood cells in the blood.The student will develop skills for teamworking.He/She will learn how to perform a laboratory report .
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes ,there will be a lecture from the tutor
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
There are no special skills required from the student. Good command of English is necessary. Previous experience with working in laboratory is a plus
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
- Hemoglobin-Based Oxygen Carrier Microparticles: Synthesis; Properties; and In Vitro and In Vivo Investigations Yu Xiong; Axel Steffen; Kristin Andreas; Susanne Müller§; Nadine Sternberg†; Radostina Georgieva; and Hans Bäumler
- Nonvasoconstrictive Hemoglobin Particles as Oxygen Carriers Yu Xiong†; Zi Zhao Liu; Radostina Georgieva; Kathrin Smuda; Axel Steffen; Mauricio Sendeski; Andreas Voigt; Andreas Patzak; and Hans Bäumler