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Gene expression analysis of non-invasive and muscle-invasive bladder tumours for the characterization of aggressive tumour phenotype
Bulgaria (AMSB) - Medical University of Sofia, Sofia
Department of Medical genetics, Medical faculty, Medical university of Sofia
Corr.memb. Draga Toncheva,MD,DSc
Type of Research Project
- Basic science
What is the background of the project?
Aging of the world population, industrialization and the increased exposure to adverse environmental factors (arylamines, aluminium, etc.) place uroepithelial tumours in the group of the socially significant diseases of modern society. The molecular pathogenesis of superficial non- invasive carcinomas differs from that of muscle- invasive bladder carcinomas. The group of muscle- invasive tumours are characterized by genetic instability and microstructural aberrations, undetectable by routine diagnostic methods. Tumours with the ability to penetrate through the basal epithelial membrane should be perceived as aggressive lesions, with certain genetic changes in this group being associated with tumour progression.Thus discovery of specific genetic markers for invasive potential and tumor progression in uroepithelial may improve the diagnosis, prophylaxis, therapy and quality of life.
What is the aim of the project?
● To characterize the expression profiles of Messenger Ribonucleic acid (mRNA) in non-invasive and muscle-invasive bladder tumours to identify prognostic markers associated with an aggressive phenotype. ● To enrich the clinical database, Deoxyribonucleic acid (DNA) and tissue biobank from the bladder tumor samples.
What techniques and methods are used?
●Extraction of DNA from the tumour samples by phenol-chloroform extraction method. ● Complementary Deoxyribonucleic acid (cDNA) preamplification of the tumour samples from the pTa, pT1, pT2, pT2a and pTb stages. ● Gene expression assay of the individual bladder cancer samples, isolated from non- invasive (pTa and pT1) and muscle-invasive (pT2, pT2a and pTb) uroepithelial tumors. ● Statistical processing and data analysis.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be expected to take active part in the research project. This will include review of the literature on their own, participation in scientific discussions and seminars and lab work. The student will get the chance to participate in DNA isolation,. Reverse transcription, take part in setting on Quantitative Polymerase chain reaction Statistical processing and data analysis are required. Written report and oral presentation on the objectives, methodology and results of the student’s work are recommendable.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, preliminary seminars on: methods and procedures for DNA extraction
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Being familiar with general rules for good laboratory practice Subjects passed: Genetics, Medical genetics
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields
- Thykjaer; T.; et al.; Identification of gene expression patterns in superficial and invasive human bladder cancer. Cancer Res; 2001. 61(6): p. 2492-9.
- Dyrskjot; L.; et al.; Identifying distinct classes of bladder carcinoma using microarrays. Nat Genet; 2003. 33(1): p. 90-6.
- Sanchez-Carbayo; M.; et al.; Molecular profiling of bladder cancer using cDNA microarrays: defining histogenesis and biological phenotypes. Cancer Res; 2002. 62(23): p. 6973-80.
- Volanis; D.; et al.; Molecular mechanisms in urinary bladder carcinogenesis. J BUON; 2011. 16(4): p. 589- 601.
- van Tilborg; A.A.; et al.; Selection of microsatellite markers for bladder cancer diagnosis without the need for corresponding blood. PLoS One; 2012. 7(8): p. e43345.
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