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Study on regulation of neoangigenesis in advanced laryngeal cancer
Bulgaria (AMSB) - Medical University of Sofia, Sofia
Molecular Medicine Center, Department of Medical Chemistry and Biochemistry, Medical University of Sofia,
Academician Prof. Vanio Mitev, MD, PhD, DSc
Assoc. Prof. Radka Kaneva, PhD; Darina Kachakova – Yordanova, PhD, Silva Giragosyan, PhD student, Veronika Petkova, PhD student
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Neovascularization is one of the specific hallmarks of a malignant neoplasm. Our work in the field of angiogenesis in laryngeal cancer has displayed unique for this type of tumor characteristics. We expect that by examining the microRNA(micro ribonucleotide acid) profile of the laryngeal cancer, we would be able to reveal part of the role of microRNAs in the regulation of neovascularization. An additional focus of our research is the lack of Hypoxia-Inducible Factor (HIF)-1 (HIF “switch”) in laryngeal cancer and the regulatory role of microRNAs in this phenotype of neovascularization. We expect to prove that there is heterogeneity in the profile of neoangiogenesis in laryngeal cancer depending on its depth in relation to the oxygen-rich lumen of the larynx.This is important for the development of new therapeutics for the inhibition of tumor neovascularization and for determining the relevance of neoangiogenesis and levels of hypoxia in tumor for its response to combined chemo-and radiotherapy.
What is the aim of the project?
Our aim is to investigate specific mechanisms of the regulatory framework of neo- angiogenesis in advanced laryngeal cancer.
What techniques and methods are used?
Laryngeal tissue maintains and biobanking, isolation of total RNA ( Ribonucleotide acid ) from tissue, Reverse Transcriptions methods, Quantification of isolated RNA, Real Time quantitative polymerase chain reaction (qPCR), Statistical Package for the Social Sciences (SPSS) statistical analysis and interpretation of results.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be expected to take active part in the research project. This will include review of the literature on his/her own, participation in scientific discussions and seminars, lab work and analysis of the results under supervision. The student will be able to participate in laboratory experiments as DNA ( deoxyribonucleic acid) and RNA isolation, using reverse transcriptase, setting up PCR ( polymerase chain reaction ) when appropriate. Written report and oral presentation on the objectives, methodology and results of the student’s work are recommended.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings, discissions with the supervisors and lab training.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student would need to have taken university level courses in genetics and biochemistry and/or molecular biology. Good command of the English language is a must, as well as basic computer skills (Windows OS, Microsoft Office – Word, Excel, Power Point). Experience with the most common molecular biology databases, e.g. PubMed, Online Mendelian Inheritance in Man (OMIM), The Exome Aggregation Consortium (ExAC) and Ensembl, is a plus. Subjects passed: university level courses in genetics, epigenetics and biochemistry and/or molecular biology Previous experience with: molecular genetic techniques and use of most common molecular biology databases, e.g. PubMed, OMIM, Ensemble etc., is a plus
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Popov TM; Goranova T; Stancheva G; Kaneva R; Dikov T; Chalakov I; Rangachev J; Konov D; Todorov S; Stoyanov O; Mitev V (2015) Relative quantitative expression of HIF-1α; HIF-2α; HIF-3α and VEGF-A in laryngeal carcinoma. Oncology Letters 9: 2879-2885
- Popov TM; Stancheva G; Goranova TE; Rangachev J; Konov D; Todorov S; Stoyanov O;Kaneva RP; Popova D (2016) Strong Correlation Between mRNA Expression Levels of HIF-2α; VEGFR1; VEGFR2 and MMP2 in Laryngeal Carcinoma. Pathol Oncol Res. 201622(4):741-6
- Popov T.M.; Stancheva G; Giragosyan S; Stoyanov O; Valcheva S; Tsenev I; Kaneva R.P.;Popova D. Correlations between PKM2; HIF-1α; c-Myc and p53 mRNA expression levels in laryngeal carcinoma. Cr Acad Bulg Sci; 2017
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