Projects
Name
Nanoparticles exposure and biomarkers for genotoxic and epigenetic changes.
University
Belgium (BeMSA) - KU Leuven, Leuven
Domain
Public Health
Departement
Environment and Health
Head
Peter Hoet
Tutor
Prof. Godderis, Prof. Hoet, Ghosh Manosij
Languages
English, Dutch, French
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
With the expanding market and increase in production capacity, concerns have been raised regarding human exposure and safety of carbon nanotubes (CNT). There are a lot of particles in the air we breathe in, some are harmless, others can be carcinogenic. It is important to know what particles are potentially harmful for us and which ones we should avoid having contact with them. This is a problem that not only concerns scientists but also everyday people. More and more people are concerned about the effect of polluted air on our health. We want to study the epigenetic effects of particles that are commonly found in the air and whether they can influence our health situation.
What is the aim of the project?
To study the effect of particles on the genome and epigenetic changes and the repercussions on our health.
What techniques and methods are used?
Carbon nanotube, Liquid chromatography–mass spectrometry (LCMS), High-performance liquid chromatography (HPLC), pyro sequencing, Polymerase chain reaction (PCR), micronucleus essay, comet essay and cytotoxicity essay will be used to analyse the samples and to determine the effect of the different materials on its direct environment. Given the relatively short period of intership, data will be analysed by using basic descriptive analytical methods: parametric or non-parameteric depending on the distrubion of the data. We do not expect that students perform complex statistical analysis.
What is the role of the student?
- The tasks will be done under supervision
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student will be involved in recruiting, exposure analysis at the site and in the lab and laboratory analysis of the samples using carbon nanotubes. The student will be able to learn the used techniques (Carbon nanotube, Liquid chromatography–mass spectrometry (LCMS), High-performance liquid chromatography (HPLC), pyro sequencing, Polymerase chain reaction (PCR), micronucleus essay, comet essay and cytotoxicity essay) under supervision of the tutor.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No, Preliminary reading by the student
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It is an advantage if the student knows some basic lab skills, but this is not obligatory. The student should have a basic understanding of the genome and epigenetic changes. Subjects passed: basic understanding of the genome and epigenetic changes (genetics)
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Students in biomedical fields
Articles
- Désiron; H et al. What is the value of occupational therapy in return to work for breast cancer patients? A qualitative inquiry aomong experts. European Journal of Cancer Care 2015; 24 (2); art.nr. doi:10.1111/ecc.12209; 267-280.
- Stocks; S et al.Trends in incidence of occupational asthma; contact dermatitis; noise-induced hearing loss; carpal tunnel syndrome and upper limb musculoskeletal disorders in European countries from 2000 to 2012. Occupational and Environmental Medicine ; 72 (4); art.nr. oemed-2014- 102534; 294- 303.
- Pauwels; S.et al. Reproducibility and validity of an FFQ to assess usual intake of methyl-group donors. Public Health Nutrition 2015; art.nr. 10.1017/S1368980014003140; 1-10.
- Tabish; A.; Baccarelli; A.; Godderis; L.; Barrow; T.; Hoet; P.; Byun; H. (2015). Assessment of Changes in Global DNA Methylation Levels by Pyrosequencing® of Repetitive Elements. Methods in Molecular Biology; 1315; art.nr. 10.1007/978-1-4939-2715-9_15; 201-7.
- Godderis; L.; Schouteden; C.; Tabish; A.; Poels; K.; Hoet; P.; Baccarelli; A.; Van Landuyt; K. (2015). Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers. Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers.; 2015; art.nr. 10.1155/2015/845041; 845041.