(Bucharest) Imunophenotypic analysis and monitorisation of hematologic neoplasia by flow cytometry
Romania (FASMR) - University of Medicine and Pharmacy "Carol Davila", Bucharest
Center of Hematology and Bone Marrow Transplant Fundeni
Prof. Dr. Daniel Coriu
Dr. Codruta Popa
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes Yes No No No
Type of Research Project
- Basic science
What is the background of the project?
Center of Hematology and Bone Marrow Transplant Fundeni (CHBMTF) is the oldest and largest hematology center in Romania. It is affiliated to international scientific groups that work in the field of diagnosis and therapy: EBMT (European Group for Blood and Marrow Transplantation), European LeukemiaNet, ESH (European Society of Hypertension), International Society of Amyloidosis. 80% of patients admitted to Fundeni Clinical Institute have malignant diseases: acute and chronic leukemia, lymphomas, Hodgkin disease, multiple myeloma, amyloidosis. For the doctors, flow cytometry has become an indispensable tool in the diagnosis of hematologic malignancies. This fact is attributable in part to the development of smaller, user-friendly, less-expensive instruments and a continuous increase in the number of clinical applications.
What is the aim of the project?
The purpose of this project is to help the doctors gain a better insight into these complicated health disorders with flow cytometry, which is routinely used in the diagnosis of blood cancers. The use of flow cytometry in the clinical laboratory has grown substantially in the last years to a point that it has become indispensable in most laboratories. In addition, this project will focus not only on the use of this technique in the differential diagnosis of a particular disorder, but also correlate immunophenotypic, molecular, and cytogenetic data in the delineation of biologically important subgroups. It offers a combined approach to the daily practice of hematology-oncology and hematopathology.
What techniques and methods are used?
1. Diagnosis and monitoring (assessment of minimal residual disease) in treatment patients with hematologic diseases (leukemia and lymphomas) 2. Monitoring harvesting and quality control of hematopoietic stem cell graft (CD34 + stem cell counting and graft cell subset analysis) 3. Analysis of lymphocyte subsets (immune deficiencies, control of immunological reconstitution post-transplant)
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
In the preanalytical part of marking the sample with different monoclonal antibodies marked with fluorochromes, the student is expected to observe very attentively the doctor's technique and to be able to perform the same procedure under the tutor's supervision by the end of the project. In the analytical part which includes the analysis of phenotypic expression of different leucocyte populations, the student should be able to generate and evaluate hypotheses that make testable predictions which can be discussed with the tutor. Moreover, is encouraged to engage in ongoing exchange of information, ideas and approaches with the people involved in the project to develop a plan, communicate findings and/or evaluate the validity of results
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Hematology, Cell Biology, Biophysics
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Advances in Flow Cytometry: Application to the Diagnosis of Hematologic Malignancy C. Darrell Jennings and Kenneth A. Foon