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(Timisoara) Curing cancer one step at a time using Chimeric antigen receptors (CARs) and NK cells
Romania (FASMR) - University of Medicine and Pharmacy "Victor Babes", Timisoara
Prof. Păunescu Virgil, MD, PhD
Type of Research Project
- Basic science
What is the background of the project?
Natural killer (NK) cells represent an attractive lymphocyte population for cancer immunotherapy due to their ability to lyse tumor targets without prior sensitization and need for human leukocyte antigens-matching, unlike T cells. Chimeric antigen receptors (CARs) represent a novel tool for development of adoptive immunotherapy by enhancing lymphocyte targeting and activation toward malignancies. CARs can be expressed in lymphocytes to create a large number of antigen-specific cells that upon recognition and binding to antigen-expressing tumor cells mediate cytotoxicity and cytokine production. The project proposed here will have a direct impact on the treatment of patients with currently non curable cancer in Romania and abroad. If successful, the project will result in a new, safe and effective treatment option for patients with currently unmet medical need.
What is the aim of the project?
An insight into modern digital pathology diagnosis using Immunohistochemistry techniques.
What techniques and methods are used?
The techniques used for this project are mainly focusing on one side on cell cultures and on the other side on bio-molecular techniques. Cell culture techniques: - In-vitro interaction among different immune cells (lymphocytes T,NK) - Marking the cells with fluorescent antibodies - In-vitro cytotoxicity tests (direct cell-to-cell contact or adding one cell culture medium over the other one) Biological molecular techniques: - Highlighting cell membrane markers - Highlighting intracellular markers using PCR (polymer chain reaction) or flow-cytometry - Quantitative and Qualitative analysis of the outcome after PCR – using NanoDrop™ spectrophotometer
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to do the following tasks: - pipetting solutions from a tube to another - to prepare culture media -to prepare other solutions in the eventuality they are needed - to check on a daily basis the cells on the culture media using inversed microscopy - to prepare probes for flow cytometry. This involves following the given work protocols - to sterilize the used lab instruments using the autoclave/ laboratory stove.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, some articles and some PowerPoint presentations concerning the main techniques used will be provided to the student.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
The student has to sign a confidentiality document that states reassures the confidentiality of the lab results, techniques, or any other information that might be an outcome within the CAR-NK project.
Type of students accepted
This project accepts: - Medical students
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