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Recellularization of Three-Dimensional Decellularized, Bio-Scaffold Rat Liver by Induced Pluripotent Stem Cells and Liver Neuro-glia Antigen 2-Positive Progenitor Cells. (Benha University)
Egypt (IFMSA-Egypt) - Benha Faculty of medicine, Benha
Stem Cell Unit, Research Central Lab, Benha Faculty of Medicine, Benha University
Dr. Nessrine Ebrahim Ahmed Salem
Dr. Nessrine Ebrahim Ahmed Salem
Required: English Accepted: Arabic
Type of Research Project
- Basic science
What is the background of the project?
High morbidity and mortality rates caused by end-stage liver disease increase the urge of continuous research. Egypt has the highest prevalence of liver cirrhosis due to epidemic Hepatitis C Virus (HCV), and this represents 14.7% of the 90 million Egyptian population. Liver transplantation has been the only available treatment option. On the other hand, a deficiency of accessible donor organs for transplantation every year results in the death of numerous patients sitting waiting for liver transplantation. Cell-based therapy and hepatic tissue bioengineering have been considered as alternative options for liver transplantation. The potential of decellularized liver bio-scaffolds (DLS) derived from solid organs as a three-dimensional (3D) bio-scaffold has been evoked as a promising modality in tissue engineering for achieving a functional liver, but questions still exist regarding the optimal cell population for seeding in DLS.
What is the aim of the project?
The aim of this research is to create three dimensional, bio-engineered scaffolds from whole rat livers which exhibit identical hepatic structure and function as alternative options for liver transplantation.
What techniques and methods are used?
Livers effectively decellularized utilizing a perfusion convention, the decellularization technique saves the ultrastructure of extracellular matrix elements, utilitarian qualities of the local microvasculature, the biliary system of the liver, and development variables fundamental for angiogenesis and liver recovery. At that point, the recellularization by seeding induced pluripotent stem cells (IPS) combined with liver neuro- glia antigen 2-positive progenitor cells (NG2+HPCs). Expected results: decellularized liver has all the necessary microstructure and the extracellular components to support seeded cells attachment, functional hepatic cell differentiation and recellularization of bio-scaffolds give evidence of the standard to the era of a full-sized, three-dimensional organ framework as a potential structure for liver grafts rebuilding for transplantation to treat liver failure. These outcomes speak to a critical progression in the bioengineering of entire organs. This innovation might give the important instruments to create engineered bio-scaffold.
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1- Student will be trained on dissection of rat livers and observe their decellularization by detergent perfusion with preserving the inlet and outlet of their blood supply and bile duct saving all properties of stroma and adhesive molecules. 2- Students will observe the bio-scaffolds recellularization with induced pluripotent stem cells and hepatic progenitors. 3- Student will learn how to deal with the bioreactor system. 4- Student will learn the basics of tissue culture and observe the culture media perfusion of the bioengineered livers.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The project includes many workshops about regenerative medicine and molecular biology.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Data analysis, and searching the web and databases.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- The use of whole organ decellularization for generation of vascularized liver organoid. Babista et al.; 2010 Oct DOI: 10.1002/hep.24067
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