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Gene Polymorphism and Expression Studies Related to Psoriasis and Acne Vulgaris Diseases in Qalyubia Governorate (Benha University)
Egypt (IFMSA-Egypt) - Benha Faculty of medicine, Benha
Molecular Biology and Biotechnology Unit, Faculty of Medicine, Benha University.
Prof. Amal Idris Ali
Prof. Naglaa Fathy Alhusseini
Required: English Accepted: Arabic
Type of Research Project
- Basic science
What is the background of the project?
Psoriasis is a chronic inflammatory skin disease with an immunogenetic background. Acne vulgaris also is a multifactorial disease of the skin. Recently several studies demonestrated that these diseases are with a strong genetic basis. Some related genes polymorphism and expression correlate with succeptability, pathogenesis and severity of these diseases. Apolipoprotein E (APOE) gene variants have been reported to influence Psoriasis risk. Several factors, including increased sebum production, and extracellular matrix (ECM) remodeling, are thought to be associated with the pathogenesis of acne. The remodeling of the ECM is regulated by a balance between matrix metalloproteinases (MMPs) and their inhibitors, called Tissue Inhibitors of Metalloproteinases (TIMPs). The potential association between MMP-2 (- 1306 C/T) and TIMP-2 (-418 G/C) polymorphisms and the risk for acne will be investigated in this study.
What is the aim of the project?
To investigate the Apolipoprotein E and Periostatin (rs3829365) genes polymorphism in Psoriasis; matrix metalloproteinase and angiotensin converting enzyme genes polymorphism in Acne vulgaris; in addition to gene expression of LXRa and survivine genes in Acne vulgaris.
What techniques and methods are used?
1- Restricted Fragment Length Polymorphism 2- Relative quantitative method for gene expression of by real-time Polymerase Chain Reaction Tools : 1. Real time polymerase chain reaction machine (Applied Biosyste7900 and stepOne) Applied Biosystem. 2. Thermocycler Verti. 3. Gel electrophoresis. 4. Gel documentation system.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The student will mainly observe
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1. Student will learn how to deal with samples. 2. Student will be trained on anti- contamination measurements. 3. Student will observe extraction of DNA and RNA. 4. Student will know about Conventional PCR program and real-time PCR program. 5. Student will know about gel electrophoresis. 6. Student will share in interpretation of results. 7. Student will share in data collection. 8. Student will share in writing a scientific paper.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lecturers in molecular biology
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Biochemistry
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Yaykasli; K. O.; Turan; H.; Kaya; E.; & Hatipoglu; O. F. (2013). Polymorphisms in the promoters of MMP-2 and TIMP-2 genes in patients with acne vulgaris. International Journal of Clinical and Experimental Medicine; 6(10); 967–972.
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