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Role of Р2-receptors in human placental trophoblast invasion
Russia (TaMSA-Tatarstan) - Kazan State Medical University, Kazan
Department of Pathology, Russia, Kazan, Orenburgskiy tr., 138
Type of Research Project
- Clinical Project without Laboratory work
What is the background of the project?
Development of the human placenta is critical for embryonic development and successful pregnancy outcome. Immediately after implantation, trophoectodermal cells forming the outermost epithelial layer of the blastocyst give rise to diverse trophoblast cell types. Cells of tumors and trophoblast are very similar. They both have the high ability to invasion. Cancer cells have been found to actively synthesize large quantities of specific proteins - receptors to TNF R1 and R2, bind to tumor necrosis factor (TNF). Normal cells also synthesize these receptors. Due to their presence on their surfaces outside the cell, it receives signals from the immune system cells that control its life cycle and capacity. Expression of the tumor necrosis factor receptor-1 (TNF-R1) gene in placental trophoblast cells is constitutive whereas expression of the TNF-R2 gene is developmentally programmed. But the main difference is that trophoblast cell do not do metastasises.
What is the aim of the project?
Comparative estimation of change of an expression of P2-receptors
What techniques and methods are used?
The method of indirect immunofluorescent detection is a technique that not only allows the detection of antigens, but also enables determination of their morphological localization and the spatial relationships of different antigens to each other. It is a valuable tool for research in the field of implantation and placentation, in which interrelation- ships between antigens and their coexpression are more important than their mere sensitive detection. A general IHC protocol consists of four main steps: (1) fixation – to keep everything in its place (2) antigen retrieval – to increase availability of proteins for detection (3) blocking – to minimize pesky background signals (4) antibody labeling and visualization – getting the pretty pictures. Indirect IF uses two antibodies. The primary antibody is unconjugated and a fluorophore-conjugated secondary antibody directed against the primary antibody is used for detection.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will take active part in the practical aspect of the project. During the laboratory work the student will be taught basic techniques of the method of indirect immunofluorescent detection, which has four main steps : (1) fixation – to keep everything in its place (2) antigen retrieval – to increase availability of proteins for detection (3) blocking – to minimize pesky background signals (4) antibody labeling and visualization – getting the pretty pictures. The method of indirect immunofluorescent detection will be performed under supervision after clear and detailed explanation. Also, student will be highly involved in the analysis of the final results.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There is theoretical teaching provided
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare an abstract - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- B.A.Ziganshin1; A.A.Spasov2; A.P.Ziganshina1; R.K.Dzhordzhikiya1; A.U.Ziganshin. ROLE OF P2 RECEPTORS IN VASCULAR TONE REGULATION. Kazan State Medical University. 2016;97(3):414-421. doi: 10.17750/KMJ2016-414
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