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[Gomez Palacio] Chronic exposure of heavy metals in the pediatric population of Coahuila, Mexico.
Universidad Juarez del Estado de Durango, Campus Gomez Palacio
Research Laboratory of the Facultad de Ciencias de la Salud Universidad Juarez Del Estado de Durango (FACSA UJED)
Dr. Gonzalo García Vargas
Dr. Gonzalo García Vargas
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The Lagunera Region, located in the central part of the North of Mexico, presents many health conditions in children resulting from chronic exposure to arsenic and lead. Latin America's largest non-ferrous metallurgical complex is located in the city of Torreon, in the State of Coahuila, where previous studies have shown that the inhabitants of the area have elevated levels of lead in the blood. The clinical conditions, present in chronic childhood patients, are mainly gastrointestinal, such as diarrhea, nausea and vomiting, among others. And at the level of the skin, mainly with erythema, papules, ulcers. In recent years, some studies also show a close relationship between academic deficit and prolonged exposure to these metals. The determination of lead in blood is by the chelation-extraction / atomic absorption spectrophotometry method.
What is the aim of the project?
To define what are the measures that should be taken in the community to reduce the impact of heavy metals in children and verify if the measures taken are correlated with one minor in public health impact.
What techniques and methods are used?
Purification of organic substances in the urine and water by means of superheating and acidity test. Use of the atomic absorption spectrophotometer to measure the levels of arsenic in urine and water. Analysis of results. Purification of organic substances in urine and water by means of overheating and acidity test. First step, add to the urine or water acetic acid and heat it to 40 degrees Celsius, for elimination of the organic substances of both substances, for 15 minutes, then cool down. The second step is the use of the atomic absorption spectrophotometer to measure the levels of arsenic in the urine, in this, it will be placed in the cell, the substance and will be introduced into the device with the corresponding absorbency care. and the third step is the analysis of results, where they are emptied into a database, and checked by hypothesis test T
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Basic management of urine and water purification will be performed on his/her own. The use of the spectrophotometer and the analysis of results will be done under supervision of the tutor. The student is expected to know the basic management of laboratory instruments and the basic handling of materials such as pipettes. The basic management of urine and water purification will be carried out by itself, through the application of acetic acid, and overheating at 40 degrees Celsius, using pipettes with a bulb. The use of the spectrophotometer and the analysis of results will be carried out under the supervision of the tutor. The student will handle the basic laboratory materials (pipettes, glasses, lighters, among others). The student will complete the database in excel by itself. The student will manage the hypothesis and statistical test programs under the supervision of the tutor.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, Readings provided by the researcher and management of databases and equipment by laboratory workers.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge on management and work in scientific laboratories, basic knowledge of chemistry, basic knowledge of physiology, basic knowledge of biology. The student is expected to make a scientific report of their experiences in research
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Caballero-Gutiérrez P; Carrillo-Rodriguez J; Gomez-Ugalde R; Jerez-Salas M. Presencia de arsénico en pozos y en cultivos en Oaxaca; México. Agronomía Mesoamericana. 2009;21(1):177.
- Becker A; Axelrad D. Arsenic and type 2 diabetes: commentary on association of inorganic arsenic exposure with type 2 diabetes mellitus: a meta-analysis by Wanget al. Journal of Epidemiology and Community Health. 2014;68(5):393-395.
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