Role of Androgens in Prostate Cancer Progression
Seconda Universita' Agli Studi Di Napoli
Pathology Department, A.O.U. Policlinico Vanvitelli
Antimo Migliaccio
Antimo Migliaccio Antonio Bilancio,
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes No No Yes Yes Yes No No Yes Yes Yes No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
This is a clinical labwork to find out exactly the influence that the androgens have in prostate cancer progression. As a result, prostate cancer (PCa) is the leading diagnosed malignancy in men in western countries. Plasma levels of androgens show variations between different populations, and in many cases this correlates with PCa susceptibility. Indeed, exposure of the fetus to higher androgen concentrations appears to be a risk factor for PCa. The androgen receptor (AR) is present in the majority of PCa, and its activation by androgens leads to different proliferative, apoptotic and angiogenic events. These events are in turn mediated by dysregulation of cyclin-dependent kinases, apoptotic factors and even mutations in the AR. Thus, an understanding of the cellular mechanisms involved in androgen action may lead to better therapeutic targets for PCa. Considering that the study began some years ago they will probably be in the last phase of the study so the student could have a very motivating result after his experience in this lab even because they use several lab techniques that the medical students normally study during their career.
What is the aim of the project?
If the correlation between the androgens and the prostate cancer (and exactly in which way) is confirmed, with this study they will be able to know in which quantity the hormones can be involved in the progression of the cancer.
What techniques and methods are used?
In this lab they will use ELISA (Enzyme-Linked Immunosorbent Assay), Cytofluorimetry, Western blot.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
At the beginning the student will only be able to see how he/she will work in the lab, then he/she will perform bench works trying to understand every step and every technique used by the tutor such as: RT (Reverse Transcription) of First Strand cDNA; PTK/PTP (Protein Tyrosine Kinase/Protein Tyrosine Phosphatases) Amplicon Amplification by Polymerase Chain Reaction. He/she will also try to do these techniques on his own but always under the supervision of the tutor to avoid accidents. His/her work will be safe anyway because the tutor takes always the right precautions.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No, there won't be any theoretical teaching provided for the student.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
He should be very motivated in order to learn how to work in a lab and improve what he knows about it. Subjects passed: General pathology and Pathological Anatomy.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- G.N. Brooke and C.L. Bevan “The Role of Androgen Receptor Mutations in Prostate Cancer Progression”