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Elucidating the molecular mechanisms linking organelle dysfunction to metabolic diseases
Korea (KMSA) - Wonkwang university, North Jeolla province
Department of Microbiology
Type of Research Project
- Basic science
What is the background of the project?
Due to functional association among the subcellular organelles, a gene mutation in a subcellular organelle leads to various clinical symptoms manifested in the nervous, skeletal and digestive organs. Recent research focus has been made on the role of a single organelle which is then elaborated into a cellular task to understand functional organelles that harmonize metabolic and energetic needs in response to various stimuli in a given tissue or organ. However, much effort is still necessary to define the precise role of the organelles implicated in metabolic diseases in humans.
What is the aim of the project?
The aim of the project is to reveal how changes in morphology or quantity of a given organell impact on cellular physiology and what drives such changes. Using the CRISPR/Cas9 gene knockout approach, we will be anayzing KO phenotypes at the level of cell, tissue and organism, and also be looking for integrated roles of the subcellular organelles in human health and diseases.
What techniques and methods are used?
Cloning, quantitative real-time PCR, western blot, in situ hybridization, confocal microscopy coupled with immunostaining, immunohistochemistry, etc. The project is a multidisciplinary work to analyze phenotypes of KO animals in relation to those in humans.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
(S)he is expected to perform basic molecular biology work such as subcloning of a gene in an expression vector, and cell biology work including immunostaining to determine subcellular localization and trafficking of expressed proteins and immunohistochemistry to determine pathological progression of specimens generated from knockout animals. These tasks are routinely performed by the student under supervision.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, research articles will be provided to be read, and senior graduate students working on similar projects are readily available for hands-on teaching. General questions will be answered in the laboratory.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Any previous lab experience is preferred but not required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Metabolic functions of peroxisomes in health and disease Wanders R.J.; Biochimie; 2014; 98:36-44.
- ATM functions at the peroxisome to induce pexophagy in response to ROS. Zhang J. et al.; Nature Cell Biology; 2015; 17(10):1259-1269
- The different facets of organelle interplay-an overview of organelle interactions. Schrader M.; et al.; Frontiers in Cell abd Developmental Biology; 2015; 3:56.
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