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Molecular subtyping of colorectal cancer using BRAF V600E , MLH1 and MSH 2 immunostaining (Menoufia University)
Egypt (IFMSA-Egypt) - Menoufia University, Menoufia
Prof Dr / Hayam Aiad
Dr. Nansy Assad - Dr. Hala said - Dr. Rania Abdallah - Dr. Mona Kora - Dr. Lamia Sabry
Type of Research Project
- Clinical Project without Laboratory work
What is the background of the project?
Colorectal cancer is the 2nd most common malignant cancer after breast cancer , It is heterogeneous disease which has variable clinical , pathological and molecular features . Molecular classification of colorectal cancer provide important information in Colon cancer with implication for patient management. Colorectal cancer (CRC) is not a single disease. Rather, CRC encompasses a heterogeneous complex of diseases, and each CRC patient has a unique disease that has been caused by distinctive genetic/epigenetic background. Theoretically, every CRC arises and behaves in a unique fashion that is unlikely to be exactly recapitulated by any other CRC. Nonetheless, we believe that tumors with similar characteristics most likely arise or behave in a similar way. The purpose of tumor classification is to find similar characteristics among individual tumors, to predict empirically the pathogenesis and biological behavior of a particular tumor.
What is the aim of the project?
The aim of the study is to evaluate immunohistochemical expression of BRAF V600E , MLH1 and MSH2 in colorectal cancer patients for stratification of them into distinct subgroups for peculiar diagnosis and future target therapy
What techniques and methods are used?
Tissue samples : - 1 ry tissue samples were collected from Menofia University teaching hospital patients with colorectal cancer for Immunohistochemical analysis, we used archival formalin, fixed paraffin embedded tissue from these patients. - Hematoxylin and eosin stained section from each case were reevaluated histopathologically by two investigators Dr Hala Said and Dr Rania Abdallah Immunohistochemistry : - (IHC) refer to process of selectively imaging antigens (eg proteins ) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1- The student will assist in the whole methods of the research which includes the tissue sampling, the immunohistochemistry and the statistical methods. 2- The student will have to attend at the pathology department for six hours for four days each week. 3- The student will write down all the above mentioned steps in his/her logbook to be signed by the tutors at the end of each shift. 4- At the end of the clerkship the student will make the conclusion of it according to the statistical studies they already made through the whole month to evaluate evaluate immunohistochemical expression of BRAF V600E , MLH1 and MSH2 in colorectal cancer patients for stratification of them into distinct subgroups for peculiar diagnosis and future target therapy
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation - The student will prepare a scientific report - The student will prepare an abstract - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
- basic knowledge in research - communication skills
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system
- Annals of Oncology; Volume 20; Issue 1; 1 January 2009; Pages 84–90
- Eric R. Fearon; John M. Carethers Molecular Subtyping of Colorectal Cancer: Time to Explore Both Intertumoral and Intratumoral Heterogeneity to Evaluate Patient Outcome Gastroenterology; Volume 148; Issue 1; January 2015; Pages 10-13
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