Characterization of gastrointestinal motility and visceral sensitivity in streptozotocin-induced diabetic rats
Spain (IFMSA-SPAIN)-University of Rey Juan Carlos, Madrid
Basic health sciences
Carlos Goicoechea
Raquel Abalo
English, Spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No Yes Yes No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Diabetes is a disease that produces lots of complications, including those related to the gastrointestinal tract. Motor function is uncoupled and there are some indications of altered visceral sensitivity as well. In laboratory animals it is common to induce the destruction of pancreatic beta-cells by administration of streptozotocin (STZ). This reproduces not only hyperglycaemia, together with polyphagia, polydipsia and polyuria, but also the complications of the disease. In our laboratory we have implemented new methods to study gastrointestinal motor and sensory function but these methods have never before been applied to the STZ-model of diabetes in rats.
What is the aim of the project?
To characterize the gastrointestinal complications associated to the development of type 1 diabetes mellitus induced by STZ administration in the rat.
What techniques and methods are used?
STZ (or its vehicle) will be administered on day 0. Changes in weight, food and water intake and somatic sensitivity will be measured during the development of diabetes. On the 3rd week after STZ administration, rats will be fasted overnight and glycaemia will be measured; then, in vivo colonic motor function will be evaluated using the bead expulsion test. On the 4th week, general gastrointestinal motor function will be evaluated by means of radiographic techniques. Afterwards, rats will be used for different experiments: - visceral pain recordings - in vitro colonic bead propulsion - in vitro colonic responses to intraluminal fluid (peristaltic activity) - collection of different tissue samples for histological/immunohistochemical analyses The in vivo functional studies will have been finished by the time the student arrives to the laboratory. The student will participate in the analyses of the functional recordings (visceral pain, radiographic analyses of general gastrointestinal motor function, in vitro colonic motor function) and, eventually, the histological preparations.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
In the project, the student would need a special qualification in order to perform some procedures with animals (like injecting drugs or using nociceptive tests), as determined by national and European regulations. Any other procedure (weighing the animal, recording food or water intake, recording changes in behavior, or placing the animal into a device for X-ray obtention, for example) would be performed by the student under supervision by qualified researchers. In any case, all the techniques involved in the project will be fully explained and performed with the student, so that learning is assured. The student will be fully involved in analyzing the results obtained using these techniques. Complementary studies may be proposed, including histochemical analyses of gastrointestinal preparations.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student should read some of our published papers to understand the techniques that will be used in the research project. In addition, the student will receive training in the different analyses to be performed during the stay.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
- The student will prepare a scientific report
- The student will prepare an abstract
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Enthusiasm for science, curiosity, determination, diligence, ability to work with others, interest in quality X Subjects passed: Anatomy, Physiology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Cabezos PA; Vera G; Martín-Fontelles MI; Fernández-Pujol R; Abalo R. Cisplatininduced gastrointestinal dysmotility is aggravated after chronic administration in the rat. Comparison with pica. Neurogastroenterol Motil. 2010; 22(7):797-805; e224-5. doi: 10.1111/j.1365-2982.2010.01483.x.
- Vera G; López-Miranda V; Herradón E; Martín MI; Abalo R. Characterization of cannabinoid-induced relief of neuropathic pain in rat models of type 1 and type 2 diabetes. Pharmacol Biochem Behav. 2012; 102(2):335-43. doi: 10.1016/j.pbb.2012.05.008.
- Uranga JA; Bastús S; Delkáder D; García-Cristóbal N; Leal-García M; Abalo R. Enteric neuropathy associated to diabetes mellitus. Revista Española de Patología Digestiva 2015; 107(6): 366-373. DOI: 1130-0108/2015/107/6/366-373
- Vera G; López-Pérez AE; Uranga JA; Girón R; Martín-Fontelles MI; Abalo R. Cannabinoid CB1 but not CB2 Signaling Contribute To Gastrointestinal Dysmotility Induced By The Antitumoral Drug Vincristine In The Rat. Front Pharmacol. 2017 Feb 6;8:37. doi: 10.3389/fphar.2017.00037.
- Uranga JA; García-Martínez JM; García-Jiménez C; Vera G; Martín-Fontelles MI; Abalo R. Alterations of the small intestinal wall and motor function after repeated cisplatin in the rat. Neurogastroenterology and Motility. DOI:10.1111/nmo.13047 (en prensa).
- Ramírez I; Pantrigo JJ; Sanz-Montemayor A; López-Pérez AE; Martín-Fontelles MI; Brookes SJH; Abalo R. Computer vision-based diameter maps to study fluoroscopic recordings of small intestinal motility from conscious experimental animals. Neurogastroenterology and Motility 2017. DOI:10.1111/nmo.13052 (en prensa).
- Costa M; Dodds KN; Wiklendt L; Spencer NJ; Brookes SJ; Dinning PG. Neurogenic and myogenic motor activity in the colon of the guinea pig; mouse; rabbit; and rat. Am J Physiol Gastrointest Liver Physiol. 2013 Nov 15;305(10):G749-59. doi: 10.1152/ajpgi.00227.2013.
- Costa M; Wiklendt L; Simpson P; Spencer NJ; Brookes SJ; Dinning PG. Neuromechanical factors involved in the formation and propulsion of fecal pellets in the guinea-pig colon. Neurogastroenterol Motil. 2015 Oct;27(10):1466-77. doi: 10.1111/nmo.12646.
- Barnes KJ; Beckett EA; Brookes SJ; Sia TC; Spencer NJ. Control of intrinsic pacemaker frequency and velocity of colonic migrating motor complexes in mouse. Front Neurosci. 2014 May 8;8:96. doi: 10.3389/fnins.2014.00096.