Study of hypocamphal and cortical oscillations and the participation of gabaergic neurons in experimental model of epilepsy.
Federal University of Rio Grande do Sul (UFRGS)
Dept. of Biochemistry, Rua Ramiro Barcelos, 2600 annex 21111, Porto Alegre, CEP 90035-003, RS, Brazil
José Claudio Fonseca Moreira
Maria Elisa Calcagnotto
English, Portuguese
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No Yes No No No No No Yes No No
Type of Research Project
- Basic science
What is the background of the project?
The reorganization and deficit in the GABAergic system in causes rupture in synaptic network, leading to changes hippocampal and cortical oscillations and in the coupling between the oscillations, favoring the synchronization process and epileptogenesis. To address these issues we will use in vivo Electroencephalography (EEG) recordings and in vitro patch clamp techniques in brain slices of animal models with epilepsy. The GABAergic system will be investigated by synaptic transmission in vitro (patch- clamp recordings) and morphology and distribution of interneurons using immunohistochemistry.
What is the aim of the project?
The aims of this project are to investigate changes in oscillation patterns and coupling between different frequency ranges in hippocampal and cortical regions in vivo and in vitro, and the influence of the GABAergic system in animal models with epilepsy.
What techniques and methods are used?
First step: Cortical and hipocampal Implantation of electrodes in epilepsy animal model. Second step: in vivo EEG recording Third step: Brain oscillations Analysis using MATALAB Fourth step in vitro patch clamp recording to study oscillations and GABAergic synaptic function Fifth step Immunohistochemistry with interneuronal markers
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student should: - attend the Weekly seminars (2x / week) During each seminar journal articles (in English) will be discussed and the student should read and participate of the discussion - observe the practical implantation of cerebral electrodes Graduated students will perform the cerebral electrodes implantation in rodents, and the students will observe and learn the procedure. - participate of the in vivo and in vitro electrophysiological recordings Seven to 10 days after implantation the animals will be placed in a recording room where the student would follow a graduate student involved in the project and observe the video-EEG. In addition, the student will follow a graduate student involved in the project in the manufacture of brain slices for posterior in vitro patch clamp recordings.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes. We have programmed 2 seminars a week to discuss issues related to the projects Recommended readings: Principles of Neural Science E. Kandel 5ed, ISBN-13: 978-0071390118 Rhythms of the brain. Gyorgy Buzsaki ISBN: 978-0199828234 The Synaptic Organization of the Brain, Gordon Shepherd. 5th Edition, ISBN: 9780195159561
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Dedication, Responsability, meticulous, to have knowledge of physiology (mainly neurophysiology), anatomy (mainly neuroanatomy) and Biochemistry. Able to work with experimental animal models. Subjects passed: physiology (mainly neurophysiology), anatomy (mainly neuroanatomy) and Biochemistry
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Students in biomedical fields
- Impairment of GABAergic system contributes to epileptogenesis in glutaric acidemia type I. Impairment of Epilepsia. 2017 Oct;58(10):1771-1781. doi:10.1111/epi.13862
- Guanosine Exerts Neuroprotective Effect in an Experimental Model of Acute Ammonia Intoxication. Mol Neurobiol. 2017 Jul;54(5):3137-3148. doi: 10.1007/s12035-016- 9892-4
- Neuroprotective effects of guanosine administration on behavioral; brain activity; neurochemical and redox parameters in a rat model of chronic hepatic encephalopathy. Metab Brain Dis. 2014 Sep;29(3):645-54. doi: 10.1007/s11011-014- 9548-x.