Development of novel rapid fluorescent diagnostic system for human infectious diseases
Korea (KMSA) - Wonkwang university, North Jeolla province
Infectious Diseases
Department of Infection biology
Seon-Ju YEO
English, Vietnamese
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes No No No No No Yes Yes No No No Yes
Type of Research Project
- Basic science
What is the background of the project?
As the infectious diseases such as Avian Influenza, Zika virus, Malaria, Dengue virus occurs frequently and globally, it is easy to be contagious to human. On-site monitoring is highly requested to isolate the suspicious patient right away in order to prevent the virus spreading. However, current rapid detection system is not sufficient to sense virus.
What is the aim of the project?
To improve the sensitivity of Rapid diagnostic system for infectious disease, fluorescent material will be adapted to the system, and novel anitibody will be developed against the pathogen.
What techniques and methods are used?
This study includes polymerase chain reaction to amplify the diagnostic antigen from envelop protein after culturing virus in mammalian cell. Polymerase chain reaction product is ligated with subcloning vector and cloned into E.coli-expressing vector to make recombinant antigen. After expressing recombinant antigen, pure antigen is purified and immunized into mice to make antibody. Antibody is conjugated with fluorescent materials or horse-raddish peroxydase enzyme to determine the reactivity against antigen and virus. For antibody-antigen binding, horse-radish peroxidase enzyme assay (enzyme-linked immunosorbent assay) is used for determination of reactivity in terms of limit of detection. Lower Linked open data indicates the better performance of assay. Linked open data is calculated by this To find the better condition, student-t-test or ANOVA(Analysis of Variance) will be performed for statistical evaluation.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1. Assisting the senior graduate student to prepare reagents and working together : Design the primer for Polymerase chain reaction, cell culture, extracting RNA, Performing Polymerase chain reaction, Run electrophoresis. Ligation, transformation to bacteria, Miniprep, Antigen expression in E.coli, make monoclonal antibody. Conjugation 2. Cleaning the lab room and organization: Check points for lab safety 3. Checking the materials left and ordering: recording lab works on lab note and purchasing materials 4. Analizing the experiment result: Check the right size of Polymerase chain reaction production, confrim the recombinant antigen expression by Western blot and enzyme-linked immunosorbent assay. 5. Presenting the experiment result: All data will be presented by student in at least everyweekend.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Regular journal meeting will be opened and students will be presenting for their work-relatd article.Each meeting will provide the articles to be discussed in advance.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
High motivation (enthusiasm) is essential! Students should have passed at least one of below subjects ; Biology / Biochemistry / Chemistry / Molecular biology English speaking and reading skills are mandatory
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles. Sci Rep. 2017 Aug 11;7(1):7933;
- Smartphone-Based Fluorescent Diagnostic System for Highly Pathogenic H5N1 Viruses. Theranostics. 2016 Jan 1;6(2):231-42.